SW 403

87071008, human colon, Epithelial

• Kod UNSPSC: 41106514

Właściwości

• product name: SW 403, 87071008
• pochodzenie biologiczne: human colon
• tryb wzrostu: Adherent
• kariotyp: 2n = 46, triploid
• morfologia: Epithelial
• produkty: Carcinoembryonic antigen (CEA)
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Caucasian colon adenocarcinoma

Opis linii komórkowej

Established from a grade III adenocarcinoma of the bowel of a 51 year old Caucasian female. The cells produce CEA and are tumorigenic in nude mice. Electron microscopy reveals prominent golgi apparatus.

Zastosowanie

Tumourigenicity studies

pożywka hodowlana

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin/EDTA; No CO₂; 37°C. Cells do not reach confluency but will grow in small islands.

Inne uwagi

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