SW 48

89012702, human colon, Epithelial

• Kod UNSPSC: 41106514

Właściwości

• product name: SW 48, 89012702
• pochodzenie biologiczne: human colon
• tryb wzrostu: Adherent
• kariotyp: 2n = 46
• morfologia: Epithelial
• produkty: Carcinoembryonic antigen (CEA)
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human colon adenocarcinoma

Opis linii komórkowej

The cells produce a low level of CEA: 0.6ng/1,000,000 cells in 10 days. Cells are tumorigenic in nude mice. Established from a grade IV ulcerating tumour encircling the bowel of an 82 year old Caucasian female. HLA = A32, A33, B27, B35

Zastosowanie

Tumourigenicity studies

Profil DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 11,12
D16S539: 11,13
D5S818: 10,14
D7S820: 9,10
THO1: 6,9.3
TPOX: 8
vWA: 18,20

pożywka hodowlana

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; No CO₂; 37°C.

Inne uwagi

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