G-401

87042204, human kidney, Epithelial-like

• Kod UNSPSC: 41106514

Właściwości

• product name: G-401, 87042204
• pochodzenie biologiczne: human kidney
• tryb wzrostu: Adherent
• kariotyp: 2n = 46, diploid
• morfologia: Epithelial-like
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Caucasian rhabdoid tumour (formerly classified as Wilm′s tumour)

Opis linii komórkowej

Derived from a tumour of a 3 month old male Caucasian. Highly transformed and grows in soft agar. Highly undifferentiated. G401 was originally described as a cell line derived from a Wilm?s tumour. Due to a change in the classification of such tumours, the cell line was examined by Garvin et al., 1993 and found to be more appropriately classified as derived from a rhabdoid tumour of the kidney.

Zastosowanie

Transformation and tumourigenicity studies

Profil DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 9,14
D16S539: 12
D5S818: 13
D7S820: 11,14
THO1: 8,9.3
TPOX: 8,11
vWA: 16

pożywka hodowlana

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Change medium every 4 days.

Inne uwagi

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