KG-1

86111306, human bone marrow, Myeloblast-like

• Synonim(y): KG1 Cells
• Kod UNSPSC: 41106514

Właściwości

• Nazwa produktu: KG-1, 86111306
• pochodzenie biologiczne: human bone marrow
• tryb wzrostu: Suspension
• kariotyp: 2n = 46, diploid
• morfologia: Myeloblast-like
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Caucasian bone marrow myelogenous leukaemia

Opis linii komórkowej

Derived from the bone marrow aspirate of a 59 year old male Caucasian with erythroleukemia that became acute myelogenous leukaemia. The cells form colonies in soft-agar in the presence of colony stimulating factor (CSF). KG-1 cells resemble acute myelogenous leukaemia showing considerable pleomorphism with a predominance of myeloblasts and promyelocytes. A small percentage of the cells are mature granulocytes with the occasional presence of macrophages and eosinophils. They do not express surface Ig or EBV antigens and lack specific markers for lymphocytic cells.

Zastosowanie

KG-1 has been used to extract total RNA and use them as templates to determine the 5′ end of promyelocytic leukemia zinc finger (PLZF) transcripts.

Profil DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19

pożywka hodowlana

IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Rutyna subkultury

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Inne uwagi

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