A673

85111504, human muscle, Epithelial-like

• Kod UNSPSC: 41106514

Właściwości

• product name: A673, 85111504
• pochodzenie biologiczne: human muscle
• tryb wzrostu: Adherent
• kariotyp: Near Diploid
• morfologia: Epithelial-like
• produkty: Transforming growth factor (Tramnsforming Growth Factor (TGF)) - ß
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Ewing sarcoma

Opis linii komórkowej

Derived from a 15 year old female patient. The cells form colonies on soft agar and tumours in antithymocyte serum treated mice.

The cell line A673 was originally described in 1973 as derived from a rhabdomyosarcoma, however, more recent analysis of the cells using advanced diagnostic techniques determined the cells to have characteristics of Ewing sarcoma rather than rhabdomyosarcoma. See citations for more information about this tumour status.

Zastosowanie

Tumourigenicity studies

Profil DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 8,13
D16S539: 11
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 15,18

pożywka hodowlana

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Inne uwagi

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