LIM1215 human

10092301, human colon, Epithelial

• Synonim(y): LIM1215 Cell Line
• Kod UNSPSC: 41106514
• pochodzenie biologiczne: human colon
• tryb wzrostu: Adherent
• kariotyp: Not specified
• morfologia: Epithelial
• produkty: Not specified
• receptory: Not specified
• powiązane choroby: cancer

Właściwości

• Nazwa produktu: LIM1215 human, 10092301
• pochodzenie biologiczne: human colon
• opis: Colon carcinoma from human colon
• tryb wzrostu: Adherent
• kariotyp: Not specified
• morfologia: Epithelial
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Colorectal carcinoma from human colon

Human colorectal carcinoma

Zastosowanie

For complete product information, please see ECACC

pożywka hodowlana

RPMI1640 (with 2mM L-Glutamine+25mM HEPES) + 10% FCS, Insulin 0.6?g/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10 μM

Rutyna subkultury

Optimal split ratio 1:2-1:4 (seeding density 2 x104 cells/cm²).Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

Inne uwagi

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Tony Burgess - Ludwig Institute for Cancer Research, Australia

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