BRIN-BG5

10033001, rat pancreas (islets), Epitheloid

• Synonim(y): BRIN
• Kod UNSPSC: 41106514

Właściwości

• product name: BRIN-BG5, 10033001
• pochodzenie biologiczne: rat pancreas (islets)
• tryb wzrostu: Adherent
• kariotyp: Modal chromosome number 66 up to passage 50
• morfologia: Epitheloid
• produkty: BRIN-BG5 is a stable, glucose-responsive insulin secreting beta cell line expressing insulin, glucokinase and IAPP as detected by immunocytochemistry. BRIN-BG5 cells express the GLUT2 glucose transporter.
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice

Opis

Pochodzenie linii komórkowej

A rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell line

Opis linii komórkowej

BRIN-BG5 is a hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma). BRIN-BG5 has been shown to be tumourigenic when transplanted into a SCID mouse host. BRIN-BG5 has been mycoplasma eradicated at ECACC and the stocks available for supply have undergone a further 10 passages without detection of mycoplasma.

pożywka hodowlana

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + FBS / FCS (F2442)

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:8 to 1:10, i.e. seeding at 2-4 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approximately28hrs. At confluence 105 cells/cm² can be expected.

Inne uwagi

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Oświadczenie o zrzeczeniu się odpowiedzialności

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

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