Mero-14

09100101

• Synonim(y): Mero14 Cells
• Kod UNSPSC: 41106514

Właściwości

• pochodzenie biologiczne: human lung
• tryb wzrostu: Adherent
• kariotyp: Number of chromosomes 72-75
• morfologia: Spindle shaped cells
• produkty: Cytokeratins 7, 8, 18 and 19. Epithelial membrane antigen (EMA)
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

Opis linii komórkowej

Mero-14 was derived from the pleural effusion from a male patient aged >60 with a history of asbestos exposure. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Zastosowanie

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

Profil DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 12,13
D5S818: 9
D7S820: 10,11
THO1: 8
TPOX: 9,11
vWA: 15,18

pożywka hodowlana

Hams F10 + 15% FCS + 2mM Glutamine

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 24hrs

Inne uwagi

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