293 GTP-AC-free

5011003, human kidney (embryonic), Epithelial

• Kod UNSPSC: 41106514

Właściwości

• product name: 293 GTP-AC-free, 05011003
• pochodzenie biologiczne: human kidney (embryonic)
• tryb wzrostu: Aggregates in suspension
• kariotyp: Not specified
• morfologia: Epithelial
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Embryo Kidney, serum-free

Opis linii komórkowej

The human embryo kidney cell line 293 (Sigma Catalogue number. 85120602) adapted to grow in the animal component free medium Gene Therapy Medium 3 (Sigma G9916). This cell line grows in clumps in suspension. The original 293 cell line from which this cell line was derived was transformed with sheared human Ad5 DNA.

Profil DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19

pożywka hodowlana

Gene Therapy Medium 3 (Sigma G9916) + 2mM Glutamine. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.

Rutyna subkultury

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran

Inne uwagi

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