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C9281

Sigma-Aldrich

Cholesterol Esterase from Pseudomonas fluorescens

lyophilized powder, ≥10,000 units/g protein

Synonim(y):

CE, bile salt-stimulated lipase, cholesteryl ester hydrolase, pancreatic cholesterol esterase, Sterol-ester acylhydrolase

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About This Item

Numer CAS:
9026-00-0
Numer EC enzymu:
3.1.1.13 (BRENDA, IUBMB)
Numer WE:
232-808-6
Numer MDL:
MFCD00071052
Kod UNSPSC:
12352204
NACRES:
NA.54

pochodzenie biologiczne

Pseudomonas fluorescens

Poziom jakości

200

Próba

10-30% (TCA-Biuret)

Formularz

lyophilized powder

aktywność właściwa

≥10,000 units/g protein

masa cząsteczkowa

~129 kDa

skład

Protein, ~20%

warunki przechowywania

under inert gas (argon)

metody

cell based assay: suitable

kolor

tan to brown

pH

7-9

rozpuszczalność

0.4 M potassium phosphate, pH 7.0: soluble 1.0 mg/mL

temp. przechowywania

−20°C

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Opis ogólny

Research area: Cell Signaling

Cholesterol Esterase (CE) is a glycoprotein that can be isolated from fungal species such as Candida cylindracea and Pseudomonas fluorescens. It is classified as a member of the lipase/esterase family and functions as a homo-dimeric protein. CE is produced in the pancreas and is released in an active form upon stimulation by Cholecystokinin (CCK).

Zastosowanie

Cholesterol esterase from Pseudomonas fluorescens has been used in:
  • in cholesterol esterase assay to quantify total cholesterol from human blood serum samples
  • a study to investigate the nondenaturing protein electro transfer of the esterase activity of lipolytic preparations
  • an optimization study of components in enzymatic cholesterol reagents containing cholesterol oxidase
  • for the modification of human plasma low-density-lipoprotein (LDL) to induce endothelial cell (EC) dysfunction and monocyte (MC) adhesion in the branched tissue-engineered blood vessels (TEBVs)
  • to hydrolyze native cholesterol ester (CE) during ​filipin staining for detection of CE within the retinal frozen sections
This enzyme is widely used in the determination of serum cholesterol in diagnostic laboratories.

Działania biochem./fizjol.

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. This activity of CE plays a vital role in steroid biosynthesis or de novo steroidogenesis. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramides. The enzyme may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis..

Inne uwagi

Contains potassium phosphate and TRITON® X-100.

Definicja jednostki

One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.

Komentarz do analizy

Protein determined by biuret.
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Piktogramy

Health hazard
GHS08

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H334

Zwroty wskazujące środki ostrożności

P261 - P284 - P501

Klasyfikacja zagrożeń

Resp. Sens. 1

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
0000084944
039M4057V
11181046
047M4005V
108M4152V

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Jounghyun H Lee et al.
Advanced biology, 5(4), e2000428-e2000428 (2021-04-15)
Atherosclerosis begins with the accumulation of cholesterol-carrying lipoproteins on blood vessel walls and progresses to endothelial cell dysfunction, monocyte adhesion, and foam cell formation. Endothelialized tissue-engineered blood vessels (TEBVs) have previously been fabricated to recapitulate artery functionalities, including vasoconstriction, vasodilation
Carolina Espinosa Álvarez et al.
Molecules (Basel, Switzerland), 25(24) (2020-12-30)
Haematococcus pluvialis is the largest producer of natural astaxanthin in the world. Astaxanthin is a bioactive compound used in food, feed, nutraceutics, and cosmetics. In this study, astaxanthin extraction from H. pluvialis by supercritical fluid extraction was evaluated. The effects
David Y Hui et al.
Journal of lipid research, 43(12), 2017-2030 (2002-11-28)
Carboxyl ester lipase (CEL), previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The active site catalytic triad of serine-histidine-aspartate is centrally
Peter E Thelwall et al.
Journal of hepatology, 59(3), 543-549 (2013-04-30)
Lysosomal Acid Lipase (LAL) deficiency is a rare metabolic storage disease, caused by a marked reduction in activity of LAL, which leads to accumulation of cholesteryl esters (CE) and triglycerides (TG) in lysosomes in many tissues. We used (1)H magnetic
Stuart A Scott et al.
Hepatology (Baltimore, Md.), 58(3), 958-965 (2013-02-21)
Cholesteryl ester storage disease (CESD) and Wolman disease are autosomal recessive later-onset and severe infantile disorders, respectively, which result from the deficient activity of lysosomal acid lipase (LAL). LAL is encoded by LIPA (10q23.31) and the most common mutation associated
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Produkty

Cholesterol Esterification

Cholesterol undergoes esterification to improve transport. Cholesterol esters are more easily packaged into the interior of lipoproteins - increasing the quantity that can be readily transported in the blood stream.

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