C7354

Anti-Calbindin-D-28K (KD-15) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonim(y): Anti-CALB, Anti-D-28K
• Numer MDL: MFCD00164553
• Kod UNSPSC: 12352203
• NACRES: NA.44

Właściwości

• pochodzenie biologiczne: rabbit
• białko sprzężone: unconjugated
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• Postać: buffered aqueous solution
• masa cząsteczkowa: antigen 28 kDa
• reaktywność gatunkowa: rat, chicken, human
• rozszerzona walidacja: independent; Learn more about Antibody Enhanced Validation
• metody: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using sections of rat, human or chicken cerebellum sections; microarray: suitable; western blot: 1:1,000 using recombinant rat calbindin-D-28K
• numer dostępu UniProt: P05937
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... CALB1(793) ; mouse ... Calb1(12307) ; rat ... Calb1(83839)

Opis

Opis ogólny

Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin), is a highly conserved 28 kDa calcium binding protein, with broad tissue distribution. Itbelongs , to a family of low molecular weight calcium-binding proteins (CaBPs). Calbindin-D-28K is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial cells.

Immunogen

synthetic peptide corresponding to the C-terminal region of rat calbindin-D-28K (amino acids 185-199). This sequence is identical in the corresponding human, mouse and bovine calbindin-D-28K sequences and is highly conserved (single amino acid substitution) in chicken and frog calbindin-D-28K.

Zastosowanie

Anti-Calbindin-D-28K (KD-15) antibody produced in rabbit has been used in:

• immunocytochemistry
• western blotting
• microarray
• immunohistochemistry

Działania biochem./fizjol.

Calbindin 1 (CALB) is a calcium-binding protein. The protein may play a role in development of Purkinje cells found in heterotopias and cerebellar dysgenesias. It also acts as a very important component of intracellular homeostasis in cerebellar neurons. The progressive decrease of calbindin content in the Purkinje cells undergoes degeneration and death during paraneoplastic changes in the cerebellum. Calbindin effects on Parkinson′s disease (PD) risk displays population specificity.

Calbindin-D-28K is an important neuroanatomical marker. Despite its broad tissue distribution, it exhibits a cell-type-specific expression pattern. It has been immunocytochemically localized in selected cells in hippocampus, where it is thought to buffer intracellular calcium, or transport intramembranous calcium.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Komentarz do analizy

Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min. at RT or 37 °C) improves immunohistochemical staining.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Zwroty wskazujące rodzaj zagrożenia: H412
• Zwroty wskazujące środki ostrożności: P273
• Klasyfikacja zagrożeń: Aquatic Chronic 3
• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable