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PC253L

Sigma-Aldrich

Anti-Calbindin D-28K (Ab-1) Rabbit pAb

lyophilized, Calbiochem®

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.43

pochodzenie biologiczne

rabbit

Poziom jakości

forma przeciwciała

serum

rodzaj przeciwciała

primary antibodies

klon

polyclonal

Postać

lyophilized

zawiera

≤0.1% sodium azide as preservative

reaktywność gatunkowa

baboon, monkey, rat, human

producent / nazwa handlowa

Calbiochem®

warunki przechowywania

OK to freeze
avoid repeated freeze/thaw cycles

izotyp

IgG

Warunki transportu

ambient

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... CALB1(793)
rhesus monkey ... Calb1(677721)

Opis ogólny

Anti-Calbindin D-28K (Ab-1), rabbit polyclonal, reecognizes the Calbindin D-28K protein in rat hippocampus. It is validated for use in frozen sections and for T cell co-stimulation.
Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes the calbindin D-28K protein.
Recognizes the Calbindin D-28K protein in rat hippocampus tissue.

Immunogen

Bovine
purified bovine cerebellum calbindin D-28K protein

Zastosowanie

Frozen Sections (whole mount/vibrotome sections, 1:1000, fluorescence)

Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, see application references)

Ostrzeżenie

Toxicity: Standard Handling (A)

Postać fizyczna

Undiluted serum.

Rekonstytucja

Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution will contain ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.

Komentarz do analizy

Positive Control
Rat striatum, hippocampus, or cortex

Inne uwagi

Antibody specificity was examined in the rat striatum, cortex, and hippocampus. Detection of primary antibody with biotin-streptavidin/peroxidase reagents according to manufacturers directions. Antibody should be titrated for optimal results in individual systems.
Conde, F., et al. 1994. J. Comp. Neurol.341, 95.
Heizmann, C.W. 1993. Acta Neurobiol. Exp.53, 15.
Baimbridge, K.G., et al. 1992. Trends Neurosci.15, 303.
Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci.16, 98.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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