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C2037

Sigma-Aldrich

Cytochrome c from bovine heart

≥95% based on Mol. Wt. 12,327 basis

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Wszystkie zdjęcia(6)

About This Item

Numer CAS:
9007-43-6
Numer WE:
232-700-9
Numer MDL:
MFCD00130890
Kod UNSPSC:
12352202
NACRES:
NA.61

pochodzenie biologiczne

bovine heart

Poziom jakości

300

Próba

≥95% based on Mol. Wt. 12,327 basis

Postać

powder or crystals

masa cząsteczkowa

12327 Da

metody

cell based assay: suitable

przydatność

suitable for molecular biology

numer dostępu UniProt

P00125

Zastosowanie

cell analysis

temp. przechowywania

−20°C

informacje o genach

cow ... CYC1(512500)

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Opis ogólny

Cytochrome C is a heme protein. It is localized between the inner and outer mitochondrial membranes.

Zastosowanie

Cytochrome c from bovine heart has been used:
  • for cytochrome oxidase staining using the mice brain tissue
  • as a component of control mixture to evaluate the accuracy of tandem mass spectra in identifying peptides
  • to study its effect on the stability, transport, and retention of biochar colloids (BCs) in saturated porous media under various solution pH and ionic strength (IS) conditions
  • as a component of staining solution for the detection of cytochrome oxidase activity in the brain tissue sections of mouse
  • as a component in the assay to study its superoxide scavenging efficiency
  • as a component of serum-free DMEM media to evaluate non-specific cytochrome C reduction in astrocytes and HT-29 cells

Działania biochem./fizjol.

Cytochrome C is a key mediator of oxidative phosphorylation. It mediates the electron transport between complex III and complex IV of the respiratory chain.

Cytochrome c has been identified as an important mediator in apoptotic pathways. The release of mitochondrial cytochrome c into the cytoplasm stimulates apoptosis and is commonly used as an indicator of the apoptotic process in the cell. Investigation on the effect of Paris Saponin I (PS I) on human gastric carcinoma cell growth (SGC7901 cells) have shown an elevated level cytoplasmic cytochrome c. Results are an inhibition of proliferation in SGC7901 cells by inducing mitochondria-dependent apoptosis through cytochrome.
The ready interconversion of cytochrome c between ferrous and ferric states makes it an efficient biological electron carrier. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal link in the respiratory chain, it forms the essential electron-bridge between the respirable substrates and oxygen.

Uwaga dotycząca przygotowania

Prepared using TCA.

Inne uwagi

View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.
This page may contain text that has been machine translated.

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Wen Yang et al.
Environmental pollution (Barking, Essex : 1987), 246, 855-863 (2019-01-10)
Application of biochar in the field has received considerable attention in recent years, but there is still little known about the fate and transport of biochar colloids (BCs) in the subsurface. Natural organic matter (NOM), which mainly consists of humic
Shiyu Xia et al.
Nature, 593(7860), 607-611 (2021-04-23)
As organelles of the innate immune system, inflammasomes activate caspase-1 and other inflammatory caspases that cleave gasdermin D (GSDMD). Caspase-1 also cleaves inactive precursors of the interleukin (IL)-1 family to generate mature cytokines such as IL-1β and IL-18. Cleaved GSDMD
Stephanie Moore et al.
Journal of chromatography. A, 1476, 1-8 (2016-11-24)
Despite the developments for faster liquid chromatographic and mass spectral detection techniques, the standard in-solution protein digestion for proteomic analyses has remained relatively unchanged. The typical in-solution trypsin protein digestion is usually the slowest part of the workflow, albeit one
Nicole J Edwards et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(12), 15753-15770 (2020-10-23)
Ischemia reperfusion (IR) injury results in devastating skeletal muscle fibrosis. Here, we recapitulate this injury with a mouse model of hindlimb IR injury which leads to skeletal muscle fibrosis. Injury resulted in extensive immune infiltration with robust neutrophil extracellular trap
Maria M Shchepinova et al.
Cell chemical biology, 24(10), 1285-1298 (2017-09-12)
Mitochondrial superoxide (O2⋅-) underlies much oxidative damage and redox signaling. Fluorescent probes can detect O2⋅-, but are of limited applicability in vivo, while in cells their usefulness is constrained by side reactions and DNA intercalation. To overcome these limitations, we developed
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Produkty

Electron Transport

Learn about the four membrane-bound protein complexes that make up the electron transport chain metabolic pathway supplying energy as ATP for cellular respiration.

HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX-NP5: Affect of pH

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX: Affect of Particle Size

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

Chromatograms

application for HPLCapplication for HPLCapplication for HPLC

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