C0543

Rat Collagen Type IV

from Engelbreth-Holm-Swarm murine sarcoma basement membrane, lyophilized powder, suitable for cell culture, used for 3D hydrogel formation

• Synonim(y): Type IV collagen, EHS-collagen
• Numer CAS: 9007-34-5
• Numer WE: 232-697-4
• Numer MDL: MFCD00130825
• Kod UNSPSC: 12352202
• NACRES: NA.75

Właściwości

• Nazwa produktu: Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane, Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture
• pochodzenie biologiczne: mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)
• Poziom jakości: 200
• typ: Type IV (Miller)
• linia produktu: BioReagent
• Formularz: lyophilized powder
• opakowanie: glass bottle of 1 vial
• metody: cell culture | mammalian: suitable
• pokrycie powierzchni: 6‑10 μg/cm²
• rozpuszczalność: 0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)
• numer dostępu UniProt: B1AVK5; P02463; P08122; Q63ZW6; Q9QZS0
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• informacje o genach: mouse ... Col4a1(12826) , Col4a2(12827) , Col4a3(12828) , Col4a5(12830) , Col4a6(94216)

Opis

Opis ogólny

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Collagen is classified into several structurally and genetically distinct types. Type IV collagen is a predominant structural protein of the basement membrane. It is notably characteristic of the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma. The target cells for attachment include: epithelial cells, endothelial cells, muscle cells, and nerve cells.

Zastosowanie

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used:

• in adhesion assays.
• in hydrogel fabrication.
• to coat cell culture plates for cell differentiation.
• to treat round plastic coverslips for scanning electron microscopy (SEM) analysis in an interaction study between A. culbertsoni trophozoites and Schwann cells.

Recommended for use as a cell culture substratum at 6-10 μg/cm².

Działania biochem./fizjol.

Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules. It is also used in 2D coatings.

Inne uwagi

Native collagen

We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma-type designations with recognized collagen classification types.

Ilość

0.75 mg/vial

Uwaga dotycząca przygotowania

Chloroform treated.
Pepsin is not used in the preparation.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)