CC076

Human Collagen Type IV

from human placenta, liquid, 1 mg/mL, suitable for cell culture, used for gel formation

• Kod UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

Właściwości

• product name: Human Collagen Type IV,
• pochodzenie biologiczne: human
• Poziom jakości: 100
• Próba: 95% (Human collagen type IV, SDS-PAGE)
• Postać: liquid
• masa cząsteczkowa: 300 kDa
• producent / nazwa handlowa: Chemicon^®
• stężenie: 1 mg/mL
• metody: cell culture | mammalian: suitable
• zanieczyszczenia: <5% Human collagen types I-III, V, VI, and non-collagen proteins
• moc wejściowa: sample type epithelial cells; sample type hematopoietic stem cell(s); sample type pancreatic stem cell(s); sample type neural stem cell(s); sample type mesenchymal stem cell(s); sample type induced pluripotent stem cell(s); sample type: human embryonic stem cell(s)
• rozpuszczalność: water: soluble at 20 °C
• numer dostępu NCBI: NM_001845.4
• numer dostępu UniProt: P02462
• Specyficzność wiązania: Peptide Source: Laminin; ; Peptide Source: Nidogen
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• informacje o genach: human ... COL4A1(1282)

Opis

Opis ogólny

Human collagen type IV is a major structural element predominantly found in the basement membrane of the skin. It is a native triple helix and heterotrimeric protein containing two α1-like and one α2-like genes. Type 4 collagen forms a sheet-like extracellular matrix in multicellular tissues that exists around certain cell types like adipocytes, skeletal muscle cells, heart muscle cells, and smooth muscle cells. Human Collagen IV is extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. The Collagen type 4 was purified by using differential salt precipitation. Purity and retention of the native helical structure were controlled by SDS-PAGE and by reaction with anti-collagen type-specific monoclonal antibodies. Molecular weight: 300kDa; Solubility: 10mg/mL Product Source: Human placenta, negative for HBsAg and HIV antibodies

Zastosowanie

Collagen IV from the human placenta is suitable for use as a coating material for cell culture studies and the formation of collagen gels. Human Collagen Type IV has been used: to prepare collagen gel for the invasion assay to provide a supportive matrix for assessing the invasive capacity of monocytes in a cell culture systemas an extracellular matrix (ECM) protein in microarray analysis, to coat nitrocellulose film slidesto coat the microchannels of the P96-O2 plate, and provide a surface for the adhesion and growth of endothelial cells (hMVECs) and renal proximal tubular epithelial cells (hRPTECs) in a co-culture system

Działania biochem./fizjol.

Human collagen type IV is associated with Alport and Goodpasture′s syndrome, as well as several rheumatological and dermatological diseases including, acquired epidermolysis bullosa.

Postać fizyczna

Liquid purified protein. In 100 μL of 0.5M acetic acid solution, pH 2.5. Salt-free, no preservative.

Przechowywanie i stabilność

Store at -20ºC for up to 12 months. Avoid repeated freeze/thaw cycles.

Komentarz do analizy

Retention of native structure was confirmed by ability to form microfibrils.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable