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Key Documents

C0371

Sigma-Aldrich

Anti-Cullin-4A antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonim(y):

Anti-Cul4A

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

rabbit

białko sprzężone

unconjugated

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

primary antibodies

klon

polyclonal

Postać

buffered aqueous solution

masa cząsteczkowa

antigen ~89 kDa

reaktywność gatunkowa

human

rozszerzona walidacja

recombinant expression
Learn more about Antibody Enhanced Validation

stężenie

~1.5 mg/mL

metody

indirect immunofluorescence: 10-20 μg/mL using HeLa cells
western blot: 1-2 μg/mL using HEK-293T cells expressing human Cullin-4A

numer dostępu UniProt

Q13619

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... CUL4A(8451)

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Opis ogólny

Cullin-4 (Cul4) proteins are the core components of ubiquitin E3 ligases. Mammalian cells express two distinct but highly homologous cullin-4 proteins, cullin-4A and cullin-4B.
Cullin-4A is a protein encoded by CUL4A gene in humans.

Zastosowanie

Anti-Cullin-4A antibody produced in rabbit is used for Immunofluorescence at a concentration of 10-20mg/mL using HeLa cells and is also used for western blotting at a concentration of 1-2μg/mL using HEK-293T cells expressing human Cullin-4A.

Działania biochem./fizjol.

Cullin-4 (Cul4) proteins are components of ubiquitin E3 ligases that regulate cell cycle, genome stability, DNA replication and transcription. CUL4-based E3 ligase plays an important role for the repression of photomorphogenesis in plants (like Arabidopsis thaliana).
Cullin-4A (Cul4A) is involved in identifying specific targets for ubiquitylation. The Cul4A gene is known to be amplified and up-regulated in breast carcinoma and many other tumor types. Cul4A associates with damage-specific DNA binding proteins (DDB1 and DDB2). It functions as substrate recruiting factors for Cul4A-Roc1 ligases. Cul4A is known to ubiquitylate several target proteins including DDB1/DDB2, CDT1 (chromatin licensing and dna replication factor 1), DNA repair protein complementing XP-C cells (XPC), histone H2A, H3 and H4. In response to DNA damage Cul4A complex regulates protein stability of p53 and mouse double minute 2 homolog (MDM2)/ human double minute 2 homolog (HDM2).

Postać fizyczna

solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

DNA damage binding protein component DDB1 participates in nucleotide excision repair through DDB2 DNA-binding and cullin 4A ubiquitin ligase activity
Li J, et al.
Cancer Research, 66(17), 8590-8597 (2006)
The human homologue for the Caenorhabditis elegans cul-4 gene is amplified and overexpressed in primary breast cancers
Chen LC, et al.
Cancer Research, 58(16), 3677-3683 (1998)
Haodong Chen et al.
The Plant cell, 18(8), 1991-2004 (2006-07-18)
Repression of photomorphogenesis in Arabidopsis thaliana requires activity of the COP9 signalosome (CSN), CDD, and COP1 complexes, but how these three complexes work in concert to accomplish this important developmental switch has remained unknown. Here, we demonstrate that Arabidopsis CULLIN4
Leigh Ann Higa et al.
Cell cycle (Georgetown, Tex.), 5(1), 71-77 (2005-12-03)
The CUL4 (cullin 4) proteins are the core components of a new class of ubiquitin E3 ligases that regulate replication and transcription. To examine the roles of CUL4 in cell cycle regulation, we analyzed the effect of inactivation of CUL4
E T Kipreos et al.
Cell, 85(6), 829-839 (1996-06-14)
The gene cul-1 (formerly lin-19) is a negative regulator of the cell cycle in C. elegans. Null mutations cause hyperplasia of all tissues. cul-1 is required for developmentally programmed transitions from the G1 phase of the cell cycle to the
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