B8894
Albumin, Acetylated from bovine serum
protease free, for molecular biology
Synonim(y):
Ac-BSA, Acetyl BSA, Acetylated BSA
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About This Item
Polecane produkty
pochodzenie biologiczne
bovine
Poziom jakości
klasa czystości
for molecular biology
linia produktu
BioReagent
Formularz
solution
oczyszczone przez
heat shock fractionation
zawiera
does not contain stabilizer or preservative
pochodzenie
USA origin
stężenie
20 mg/mL in H2O
metody
FISH: suitable
zanieczyszczenia
HIV I and HIVII, HCV and HBsAg, tested negative
numer dostępu UniProt
obecność zanieczyszczeń
DNase free
DNase, RNase, NICKase and protease, none detected
NICKase free
Protease free
RNase free
temp. przechowywania
−20°C
informacje o genach
bovine ... ALB(280717)
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Opis ogólny
Serum albumin is the most extensive plasma protein in the circulatory system. It is produced within the liver and exported as a non-glycosylated protein to the blood plasma. The molecular weight of native serum albumin is around 66 kDa. Native serum albumin is a negatively charged protein.
Acetylation of bovine serum albumin particularly modifies lysine (Lys) residues with elevated frequency, with lesser degrees of modification of serine (Ser) and threonine (Thr) residues. MALDI-TOF mass spectrometry analysis of acetylated BSA has indicated an increased MW of about 3000, compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. This acetylation process also derivatizes similar amino acid residues in any trace contaminating nucleases in the BSA, to inactivate that nuclease activity. This makes acetylated BSA useful in molecular biology applications where trace nuclease activity must be minimized.
Acetylation of bovine serum albumin particularly modifies lysine (Lys) residues with elevated frequency, with lesser degrees of modification of serine (Ser) and threonine (Thr) residues. MALDI-TOF mass spectrometry analysis of acetylated BSA has indicated an increased MW of about 3000, compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. This acetylation process also derivatizes similar amino acid residues in any trace contaminating nucleases in the BSA, to inactivate that nuclease activity. This makes acetylated BSA useful in molecular biology applications where trace nuclease activity must be minimized.
Zastosowanie
Acetylated BSA has been used as a component of the reaction mixture for adapter ligation. In addition, this B8894 acetylated BSA product has been used in other applications, such as:
- In cDNA library preparation (Hafner, M. et al., RNA, 17(9), 1697-1712 (2011))
- As a blocking agent with streptavidin beads used for capture of biotinylated RNA (Winz, M.-L. et al., Nat. Protocol., 12(1), 122-149 (2017))
Acetylated to inactivate nucleases commonly found in BSA. Acetylated BSA should not be used as a protein standard since acetylation of tyrosine residues prevents color development in the Lowry assay and other similar protein determinations.
Działania biochem./fizjol.
Native serum albumin plays a major role in maintaining the pH and osmotic pressure of the blood. It is also involved in transporting a diverse range of endogenous and exogenous metabolites, such as amino acids, fatty acids, drugs, steroids and metal ions. Native serum albumin can have a role in modulating the levels of Ca2+ and Mg2+ metals in blood.
Cechy i korzyści
This acetylated BSA product has been tested for the following properties:
- DNase-free / Exonuclease-free
- Nickase-free / Endonuclease-free
- Protease-free
- RNase-free
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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