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A1054

Sigma-Aldrich

Anti-Digoxin–Alkaline Phosphatase antibody, Mouse monoclonal

clone DI-22, purified from hybridoma cell culture

Synonim(y):

Monoclonal Anti-Digoxin, Monoclonal Anti-Digoxin–Alkaline Phosphatase antibody produced in mouse

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.46

pochodzenie biologiczne

mouse

rekombinowane

expressed in mouse cell line

białko sprzężone

alkaline phosphatase conjugate

forma przeciwciała

purified from hybridoma cell culture

rodzaj przeciwciała

primary antibodies

klon

DI-22, monoclonal

Postać

buffered aqueous solution

metody

direct ELISA: 1:10,000 using using 20 μg/ml of purified digoxin-BSA for coating and pNPP substrate
direct ELISA: 1:6,000 using using 10 μg/ml of digoxigenin-transferrin for coating and pNPP substrate
dot blot: 1:20,000 using digoxigenin-labeled biomolecules (chemiluminescent)

izotyp

IgG1

Warunki transportu

wet ice

temp. przechowywania

2-8°C

docelowa modyfikacja potranslacyjna

unmodified

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Opis ogólny

Digoxin is a cardiac glycoside that is used to treat chronic heart failure.Monoclonal Anti-Digoxin-Alkaline Phosphatase antibody can be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies and peptides. The antibody is specific for digoxin and digoxin-labeled compounds. The product shows strong cross-reactivity with digoxigenin.

Specyficzność

Antibody is specific for digoxin and shows a high affinity for digoxigenin.

Immunogen

Digoxin-KLH.

Zastosowanie

Monoclonal Anti-Digoxin-Alkaline Phosphatase antibody is suitable for use in direct ELISA (1:10000-1:6000) and dot blot (at 1:20,000 dilutions using digoxigenin-labeled biomolecules). It has also been used for enzyme immunoassay (EIA).
Nested PCR products were hybridized with 5′-end digoxigenin-labeled oligonucleotide probe. ELISA assays were performed to detect the probe by adding monoclonal anti-digoxin antibody alkaline phosphatase (DI-22) diluted 1:1000 in 1 M phosphate-buffered saline, 1% bovine serum albumin, and 0.1% Tween 20 (Ph 7.4). Detection was performed using Sigma Fass-Pnpp substrate tablets.

Postać fizyczna

Solution in 0.05 M Tris buffer, pH 8.0 containing 1% BSA, 50% glycerol, 1 mM MgCl2 and 15 mM sodium azide

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Odwiedź Bibliotekę dokumentów

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Mbatha JN, et al.
BMC Research Notes, 10(1), 1-8 (2017)
J Kauppinen et al.
Journal of clinical microbiology, 37(5), 1454-1458 (1999-04-16)
Mycobacterium malmoense is an opportunistic human pathogen of increasing clinical importance. Since it is difficult to detect and identify the organism by conventional techniques, it was decided to seek a nucleic acid amplification method specific for M. malmoense. The method
J N Mbatha et al.
BMC research notes, 10(1), 702-702 (2017-12-07)
Cervical cancer is a major problem in women and it is important to find a suitable and acceptable screening method, especially among young in low-resource areas for future human papillomavirus (HPV) vaccine follow-up investigations. The study sought to test the
The New England journal of medicine, 336(8), 525-533 (1997-02-20)
The role of cardiac glycosides in treating patients with chronic heart failure and normal sinus rhythm remains controversial. We studied the effect of digoxin on mortality and hospitalization in a randomized, double-blind clinical trial. In the main trial, patients with
Liang Wang et al.
Theranostics, 11(2), 925-940 (2021-01-05)
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