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Merck

P4978

Sigma-Aldrich

Phosphatase, Alkaline from calf intestine

buffered aqueous glycerol solution

Synonim(y):

CIAP, CIP, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

Numer CAS:
Numer EC enzymu:
Numer MDL:
Kod UNSPSC:
12352204
eCl@ss:
42010105
NACRES:
NA.53

klasa czystości

for molecular biology

Formularz

buffered aqueous glycerol solution

masa cząsteczkowa

~80 kDa

stężenie

≥10,000 units/mL

numer dostępu UniProt

obecność zanieczyszczeń

DNase, RNase, none detected

Warunki transportu

wet ice

temp. przechowywania

−20°C

informacje o genach

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Opis ogólny

Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.

Zastosowanie

Commonly used to remove the 5′-terminal phosphate from nucleic acids during molecular cloning reactions.

Komponenty

Alkaline phosphatase is provided in a solution of 10 mM Tris-HCl (pH 8.2), 50 mM KCl, 1 mM MgCl2, and 0.1 mM ZnCl2, in 50% (w/v) glycerol. 10X CIP Buffer, Product Number C3225, is included.

Definicja jednostki

One unit will hydrolyze 1 μmole of p-nitrophenyl phosphate per min at 37 °C.

Inne uwagi

Alkaline phosphatase in 5 mM EDTA (pH 8) will be irreversibly heat inactivated at 75 °C for 10 minutes.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Certyfikaty analizy (CoA)

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Odwiedź Bibliotekę dokumentów

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Protokoły

CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

Test enzymatyczny fosfatazy alkalicznej, test dietanoloaminy (EC 3. 1. 3. 1)

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