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P4978

Sigma-Aldrich

Phosphatase, Alkaline from calf intestine

buffered aqueous glycerol solution

Synonim(y):

CIAP, CIP, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

Numer CAS:
9001-78-9
Numer EC enzymu:
3.1.3.1 (BRENDA, IUBMB)
Numer MDL:
MFCD00131849
Kod UNSPSC:
12352204
eCl@ss:
42010105
NACRES:
NA.53

klasa czystości

Molecular Biology
for molecular biology

Formularz

buffered aqueous glycerol solution

masa cząsteczkowa

~80 kDa

stężenie

≥10,000 units/mL

numer dostępu UniProt

P19111

obecność zanieczyszczeń

DNase, RNase, none detected

Warunki transportu

wet ice

temp. przechowywania

−20°C

informacje o genach

cow ... ALPI(280993)

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Opis ogólny

Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.

Zastosowanie

Commonly used to remove the 5′-terminal phosphate from nucleic acids during molecular cloning reactions.

Komponenty

Alkaline phosphatase is provided in a solution of 10 mM Tris-HCl (pH 8.2), 50 mM KCl, 1 mM MgCl2, and 0.1 mM ZnCl2, in 50% (w/v) glycerol. 10X CIP Buffer, Product Number C3225, is included.

Definicja jednostki

One unit will hydrolyze 1 μmole of p-nitrophenyl phosphate per min at 37 °C.

Inne uwagi

Alkaline phosphatase in 5 mM EDTA (pH 8) will be irreversibly heat inactivated at 75 °C for 10 minutes.
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Inhibitor

Numer produktu
Opis
Cennik

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D2886

DNA Ligase from T4-infected Escherichia coli, buffered aqueous glycerol solution

P4390

Polynucleotide Kinase from T4-infected Escherichia coli, 10 units/μL, buffered aqueous glycerol solution

G5663

Glutathione S-Transferase from E. coli, recombinant, expressed in E. coli, buffered aqueous solution

D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

R6513

Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

R4642

Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

P6911

Protease from Streptomyces griseus, BioReagent, DNase, RNase, and nickase, none detected (No RNase.)

LIG1

DNA Ligation Kit

substrat

Numer produktu
Opis
Cennik

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

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Certyfikaty analizy (CoA)

Lot/Batch Number
SLCF6895
SLCC6411
SLCB1417
SLBZ1130
SLBT7771

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Protokoły

Calf Intestinal (CIP) Alkaline Phosphatase for Nucleic Acid Dephosphorylation

CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

Test dietanoloaminowy do testu enzymatycznego fosfatazy alkalicznej (EC 3.1.3.1)

Test enzymatyczny fosfatazy alkalicznej, test dietanoloaminy (EC 3. 1. 3. 1)

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