10567752001

Alkaline Phosphatase

EIA grade, from calf intestine

• Synonim(y): AP, Alkaline Phosphatase
• Numer EC enzymu: 3.1.3.1 (BRENDA, IUBMB)
• Kod UNSPSC: 12352204

Właściwości

• pochodzenie biologiczne: bovine (calf) intestine
• Poziom jakości: 100
• Postać: solution
• aktywność właściwa: ~3,000 U/mg enzyme protein (+37°C, 4-nitrophenyl phosphate substrate, diethanolamine as buffer)
• opakowanie: pkg of 1.5 mL (15 mg)
• producent / nazwa handlowa: Roche
• optymalne pH: 9.8
• zakres pH: 9.0-11.0
• Warunki transportu: wet ice

Opis

Opis ogólny

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Amino groups: 8-16 moles/mole enzyme

Zastosowanie

Alkaline phosphatase can be coupled to other proteins via its amino groups. The enzyme solution can be used directly for conjugation without prior dialysis.

Jakość

Purity: single peak (HPLC)

Sekwencja

Purification procedure
AP from intestine is a dimeric glycoprotein, it is not known, whether the carbohydrate part is separated during purification procedure. Content of amino groupsIn the literature the carbohydrate content is 2 to 20 percent so one should find approx. 70 amino groups/mol.
In addition one researcher determined the amino groups and found 50 to 60 lysins/mol.-SH groups in AP
No information in literature about -SH groups in AP.

Carbohydrate structure
Results of investigation of the carbohydrate structure of several lots of alkaline phosphatase are available.

NH₂- and carbohydrate estimation
Results of NH₂- and carbohydrate estimation are available.

Definicja jednostki

Unit Conversion: Approx. 4.35 U (EIA grade AP) [37 °C, 4-NPP as substrate, diethanolamine as buffer, pH 9.8] = 1 U [+25 °C, 4-NPP as substrate, glycine as buffer, pH 10.5]
1.0 U [+37 °C, QC assay conditions] = 0.73 U [+30 °C, QC assay conditions] = 0.48 U [+25 °C, QC assay conditions]

Postać fizyczna

Solution, ready-to-use, 10mg/ml

Uwaga dotycząca przygotowania

Storage conditions (working solution): The diluted enzyme (1:10 in 3 M NaCl) will give a stability of 6 months at 4 °C.
1:10 dilution in water will give stability for 1 week at 4°C.
Do not freeze the diluted or undiluted enzyme, because freezing will destroy enzyme activity.

Przechowywanie i stabilność

Store at 2 to 8 °C. (Do not freeze)

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

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Informacja o środkach bezpieczeństwa

• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): does not flash
• Temperatura zapłonu (°C): does not flash