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Key Documents

3446-005-01

Sigma-Aldrich

Cultrex 3-D Culture Matrix Laminin I

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About This Item

Kod UNSPSC:
12352200

pochodzenie biologiczne

mouse (Engelbreth-Holm-Swarm (EHS) mouse tumor)

sterylność

sterile

Próba

≥90% (SDS-PAGE)

stężenie

6 mg/mL

metody

cell culture | mammalian: suitable

rozpuszczalność

water: miscible

Warunki transportu

dry ice

temp. przechowywania

−70°C

Opis ogólny

3-D Culture Matrix Mouse Laminin I may be used as a gel on which to grow cells, or as a media additive alone or in concert with other basement membrane components, to study cellular growth and differentiation in three dimensions in vitro. To offer the most standardized Laminin I for use in 3-D cultures, a special process is employed to provide material at a standard concentration of 6 mg/ml (by absorbance and extinction coefficient).

Postać fizyczna

Solution in Dulbecco′s Modified Eagle′s medium containing 10 μg/ml gentamycin sulfate and no phenol red.

Inne uwagi

Functional Assay:
3-D Culture: Laminin I promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) into acinar structures.
Cell Attachment: Tested for the ability to promote cell attachment and spreading of MG63 human osteosarcoma cells.
Sterility Testing:
No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP XXIV Chapter 71 sterility test.No mycoplasma contamination detected by PCR.Endotoxin concentrations = 20 EU/ml by LAL assay.
Storage Conditions and Product Stability:
Product is stable for a minimum of 3 months from date of shipment when stored at -80 °C in a manual defrost freezer. For optimal stability, store at -80 °C in aliquots. Avoid repeated freeze/thaw cycles.

Informacje prawne

3-D Culture Matrix is a trademark of Trevigen, Inc.
This page may contain text that has been machine translated.

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Daniel Rüdiger et al.
Cell reports, 32(6), 108015-108015 (2020-08-14)
Endothelial tube formation on a reconstituted basement membrane (Matrigel) is a well-established in vitro model for studying the processes of angiogenesis and vasculogenesis. However, to date, the organizing principles that underlie the morphogenesis of this network and that shape the initial
Ruilin Tian et al.
Neuron, 104(2), 239-255 (2019-08-20)
CRISPR/Cas9-based functional genomics have transformed our ability to elucidate mammalian cell biology. However, most previous CRISPR-based screens were conducted in cancer cell lines rather than healthy, differentiated cells. Here, we describe a CRISPR interference (CRISPRi)-based platform for genetic screens in

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