14533

bisBenzimide H 33342 trihydrochloride

for fluorescence, ≥97.0% (HPLC)

• Synonim(y): 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, bisBenzimide
• Wzór empiryczny (zapis Hilla): C₂₇H₂₈N₆O · 3HCl · xH₂O
• Numer CAS: 875756-97-1
• Masa cząsteczkowa: 561.93 (anhydrous basis)
• Beilstein: 1234011
• Numer WE: 245-691-1
• Numer MDL: MFCD00012678
• Kod UNSPSC: 12161505
• Identyfikator substancji w PubChem: 57647173
• NACRES: NA.25

Właściwości

• klasa czystości: for fluorescence
• Próba: ≥97.0% (HPLC)
• fluorescencja: λ_ex 340 nm; λ_em 510 nm; λ_ex 355 nm; λ_em 465 nm in TE buffer; DNA
• przydatność: suitable for fluorescence
• temp. przechowywania: 2-8°C
• ciąg SMILES: Cl[H].Cl[H].Cl[H].CCOc1ccc(cc1)C2=NCc3cc(ccc3N2)C4=NCc5cc(ccc5N4)N6CCN(C)CC6
• InChI: 1S/C29H32N6O.3ClH/c1-3-36-25-8-4-20(5-9-25)28-30-18-22-16-21(6-10-26(22)32-28)29-31-19-23-17-24(7-11-27(23)33-29)35-14-12-34(2)13-15-35;;;/h4-11,16-17H,3,12-15,18-19H2,1-2H3,(H,30,32)(H,31,33);3*1H
• Klucz InChI: FYEVKHPLBHLWHK-UHFFFAOYSA-N

Opis

Zastosowanie

Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm

bisBenzimide H 33342 trihydrochloride can be used to study biochemicals and reagents, fluorescent probes, labels, particles and stains, luminescent compounds and detection and wavelength index. bisBenzimide H 33342 trihydrochloride, Hoechst 33342, has been used in a study to investigate the effects of inorganic and organic arsenic compounds on human T-lymphoblastoid leukemia cells. Hoechst 33342 has also been used in a study to examine the effects of 9 calcium antagonists on ABCG2/BCRP-mediated resistance and transport in HeLa and SN-38-resistant HeLa (HeLa/SN100) cells, overexpressing ABCG2/BCRP.

Działania biochem./fizjol.

Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.

Opakowanie

Bottomless glass bottle. Contents are inside inserted fused cone.

Inne uwagi

Suitable for vital DNA staining of a variety of cell types; As a probe of membrane permeability in mammalian cells

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Gloves