05479
Bovine Serum Albumin
fraction V, essentially protease free, ≥92% (GE)
Synonim(y):
Albumin bovine serum, BSA, Bovine albumin
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About This Item
Polecane produkty
pochodzenie biologiczne
bovine
Poziom jakości
Próba
≥92% (GE)
Formularz
lyophilized powder
jakość
essentially protease free
masa cząsteczkowa
~66 kDa
Mr ~67000
oczyszczone przez
heat shock fractionation
strata
≤5% loss on drying
rozpuszczalność
H2O: 40 mg/mL at 20 °C, clear to very faintly turbid, colorless to slightly yellow
H2O: slightly soluble
obecność zanieczyszczeń
protease ≤0.005 U/mg
temp. przechowywania
2-8°C
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Zastosowanie
Albumin from bovine serum has been used for blocking the non-specific protein binding surface areas. It has also been used as a standard for evaluating the concentration of protein by using Bradford method.
Działania biochem./fizjol.
Bovine serum albumin also referred to as BSA or Fraction V is a protein isolated from cows. BSA can block vacant binding sites in enzyme-linked immunosorbent assay (ELISA) in both poly-l-lysine (PLL)-treated as well as non-treated microwells. It can also triggers the insulin dependent diabetes mellitus.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Uwaga dotycząca przygotowania
Initial fractionation by heat shock.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Inne uwagi
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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