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Kluczowe dokumenty
A3059
Bovine Serum Albumin
heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%
Synonim(y):
Albumin bovine serum, BSA, Bovine albumin
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About This Item
Polecane produkty
pochodzenie biologiczne
bovine
Poziom jakości
Próba
≥98%
Postać
lyophilized powder
masa cząsteczkowa
~66 kDa
oczyszczone przez
heat shock fractionation
opakowanie
poly bottle of
pochodzenie
USA origin
metody
ELISA: suitable
western blot: suitable
zanieczyszczenia
essentially globulin free
protease, none detected
strata
≤5%
pH
7
rozpuszczalność
water: soluble (40 mg/ml)
numer dostępu UniProt
obecność zanieczyszczeń
BT Virus, none detected
VSV Virus, none detected
temp. przechowywania
2-8°C
informacje o genach
bovine ... ALB(280717)
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Opis ogólny
Bovine serum albumin (BSA) has an important role in the regulation of blood colloidal osmotic pressure. BSA is the major protein in bovine serum, and is composed of 583 amino acids with a calculated molecular weight of 66,430 Daltons. BSA is used in many different applications and fields, such as:
This particular BSA is specifically evaluated and tested to have very low protease content / for protease-free status. Protease-free BSA may be used in applications like:
b) Nucleic acid hybridization
c) Radioimmunoassay (RIA)
- Protein quantitation (as a standard)
- Immunochemistry (as a blocking agent)
- Cell culture (as a nutrient)
This particular BSA is specifically evaluated and tested to have very low protease content / for protease-free status. Protease-free BSA may be used in applications like:
- Enzyme assays
- Protein-based assays
- Protease-sensitive techniques that include:
b) Nucleic acid hybridization
c) Radioimmunoassay (RIA)
Zastosowanie
Bovine Serum Albumin has been used in luminescence assays.
Działania biochem./fizjol.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Cechy i korzyści
- Heat shock fractionated
- Protease-free
- Essentially globulin-free
Uwaga dotycząca przygotowania
Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
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Numer produktu
Opis
Cennik
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
Certyfikaty analizy (CoA)
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