E9379

Stains-All

~95% purity, powder

• Synonim(y): 1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide, 3,3′-Diethyl-9-methyl-4,5,4′,5′-dibenzothiacarbocyanine
• Wzór empiryczny (zapis Hilla): C₃₀H₂₇BrN₂S₂
• Numer CAS: 7423-31-6
• Masa cząsteczkowa: 559.58
• Beilstein: 3865213
• Numer WE: 231-047-7
• Numer MDL: MFCD00078788
• Kod UNSPSC: 12171500
• Identyfikator substancji w PubChem: 24894694
• NACRES: NA.47

Właściwości

• Nazwa produktu: Stains-All, ~95%
• Próba: ~95%
• Poziom jakości: 200
• Formularz: powder
• metody: microbe id | staining: suitable
• rozpuszczalność: chloroform: 40 mg (PLUS 4.0 ml)
• Zastosowanie: diagnostic assay manufacturing; hematology; histology
• temp. przechowywania: 2-8°C
• ciąg SMILES: [Br-].CCN1C(/Sc2ccc3ccccc3c12)=C\C(C)=C\c4sc5ccc6ccccc6c5[n+]4CC
• InChI: 1S/C30H27N2S2.BrH/c1-4-31-27(33-25-16-14-21-10-6-8-12-23(21)29(25)31)18-20(3)19-28-32(5-2)30-24-13-9-7-11-22(24)15-17-26(30)34-28;/h6-19H,4-5H2,1-3H3;1H/q+1;/p-1
• Klucz InChI: MPBRYMWMMKKRGC-UHFFFAOYSA-M

Opis

Zastosowanie

Stains-All has been used for the staining of gel made for size determination of hyaluronan. It has also been used for the staining of DNA oligonucleotides separated by nondenaturing polyacrylamide gel electrophoresis.

Działania biochem./fizjol.

Stains-All is a cationic carbocyanine dye and is a multipurpose dye. It stains sialoglycoproteins blue, Ca²⁺ binding proteins deep blue to violet, proteins red and lipids yellow-orange. It also stains RNA (bluish purple) and DNA (blue).

As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.

Przestroga

Upon continued exposure to light the nucleic acid-dye complex will fade and disappear.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)