198161

Toluidine Blue O

certified by the Biological Stain Commission

• Synonim(y): Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride
• Wzór empiryczny (zapis Hilla): C₁₅H₁₆ClN₃S
• Numer CAS: 92-31-9
• Masa cząsteczkowa: 305.83
• Numer w indeksie barw: 52040
• Numer WE: 202-146-2
• Numer MDL: MFCD00011934
• Kod UNSPSC: 12171500
• Identyfikator substancji w PubChem: 24851865
• NACRES: NA.47

Właściwości

• klasa czystości: certified by the Biological Stain Commission
• Poziom jakości: 200
• Formularz: powder
• skład: Dye content, 80%
• kolor: dark green
• λmaks.: 626 nm
• ε (współczynnik ekstynkcji): ≥13000 at 241-247 nm in H₂O; ≥28000 at 287-293 nm in H₂O; ≥40000 at 625-635 nm in H₂O
• Zastosowanie: diagnostic assay manufacturing; hematology; histology
• temp. przechowywania: room temp
• ciąg SMILES: [Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C
• InChI: 1S/C15H15N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8,16H,1-3H3;1H
• Klucz InChI: GEDVVYWLPUPJJZ-UHFFFAOYSA-N

Opis

Opis ogólny

A metachromatic dye suitable for a wide variety of histological staining procedures.

Zastosowanie

Toluidine Blue O along with light-emitting diode has been used as a photosensitizer to control in vitro biofilms and to kill gram-negative bacteria.

Działania biochem./fizjol.

Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. TBO can be removed from its aqueous solution by using an effective adsorbent, clinoptilolite. TBO and fixed output laboratory laser light source are used together to destroy variety of oral bacteria associated with dental caries. This metachromatic nuclear stain is often used in place of thionin or methylene blue. It also serves as a component of a tribasic stain along with Malachite Greenand Basic Fuchsin.

Przydatność

Certified for use as a metachromatic stain for mast cells and cartilage using the Churukian-Schenk method and the method of Nocht on paraffin sections.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)