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XTGHP-RO

Roche

X-tremeGENE™ HP DNA Transfection Reagent

High-performance polymer reagent for transfecting many cell lines

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About This Item

Kod UNSPSC:
41106502
NACRES:
NA.55

klasa czystości

for molecular biology

Poziom jakości

100

Postać

liquid (aqueous solution)

zastosowanie

 mL (suitable for 165 transfections)

opakowanie

pkg of 0.4 mL (06366244001)
pkg of 1.0 mL (06366236001)
pkg of 5 × 1 mL (06366546001)

producent / nazwa handlowa

Roche

metody

transfection: suitable

temp. przechowywania

−20°C

Opis ogólny

X-tremeGENE HP DNA Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells.

Cechy i korzyści

X-tremeGENE HP DNA Transfection Reagent is a high performance reagent that transfects a broad range of eukaryotic cells, including insect cells and hard-to-transfect cell lines (e.g., Sf9, U-2 OS, MEF, MCF7, Hep G2, PC-3, HeLa, HT-29, HT-1080, K-562, RAW 264.7, Jurkat, PC-12, and HCT 116).

  • Benefit from an easy-to-use non-liposomal reagent that is free of animal-derived components, stable at room temperature, filtered through 0.2 μm pore size membrane, and active in serum-containing medium.
  • Achieve new levels of transfection efficiency in primary cells and tumor cell lines that are not transfected well by other reagents.
  • Generate physiologically relevant data using a reagent with low cytotoxic effects.
  • Increase experimental throughput and enable target evaluation using a simple and consistent protocol.

Jakość

Each lot of X-tremeGENE HP DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE HP DNA Transfection Reagent (ratio 3:1 ¼l/¼g DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Postać fizyczna

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE HP DNA Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

Informacje prawne

X-tremeGENE is a trademark of Roche
This page may contain text that has been machine translated.

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Piktogramy

FlameExclamation mark
GHS02,GHS07

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H225 - H319

Klasyfikacja zagrożeń

Eye Irrit. 2 - Flam. Liq. 2

Kod klasy składowania

3 - Flammable liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

334.4 °F

Temperatura zapłonu (°C)

168 °C

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Xin Yong et al.
Cancer letters, 374(2), 292-303 (2016-03-05)
Helicobacter pylori (H. pylori) infection is considered a major risk factor for gastric cancer. CagA behaves as a major bacterial oncoprotein playing a key role in H. pylori-induced tumorigenesis. Cancer stem cells (CSCs) are believed to possess the ability to initiate tumorigenesis
Isabelle Coupienne et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 10(12), 1868-1878 (2011-10-29)
Glioblastoma constitute the most frequent and deadliest brain tumors of astrocytic origin. They are resistant to all current therapies and are associated with a high rate of recurrence. Glioblastoma were previously shown to respond to treatments by 5-aminolevulinic acid (5-ALA)-based
Richard R W Brady et al.
Carcinogenesis, 32(7), 1069-1077 (2011-05-10)
Long-term aspirin or related non-steroidal anti-inflammatory drugs (NSAIDs) ingestion can protect against colorectal cancer (CRC). NSAIDs have a pro-apoptotic activity and we have shown that stimulation of the nuclear factor-kappaB (NF-κB) pathway is a key component of this pro-apoptotic effect.
Jihoon Kim et al.
Bioorganic & medicinal chemistry, 19(24), 7582-7589 (2011-11-11)
The signal transducer and activator of transcription 3 (STAT3) is constitutively activated in cancer cells. Therefore, blocking the aberrant activity of STAT3 in tumor cells is a validated therapeutic strategy. To discover novel inhibitors of STAT3 activity, we screened against
Ido D Weiss et al.
Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, 14(1), 106-114 (2011-02-25)
Expression of CXCR4 in cancers has been correlated with poor prognosis and increased metastasis. Quantifying CXCR4 expression non-invasively might aid in prognostication and monitoring therapy. We evaluated a radiolabeled antagonist of CXCR4, ⁶⁴Cu-AMD3100, as a positron-emitting imaging agent. CXCR4-transfected or
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Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalian cells without having to resort to classical gene knockout techniques.

Wprowadzenie do transfekcji komórek

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Introduction to Cell Transfection

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.

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Protokoły

Lentiviral Production Using X-tremeGENE HP Transfection Reagent

Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.

X-tremeGENE HP DNA Transfection Reagent Protocol

Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

Co-transfection of Plasmid DNA

Transient co-transfection of plasmids is a method that is commonly employed for cellular protein-protein interaction studies, transcription factor studies, and gene knockdown studies using shRNA encoding plasmids.

Protokoły transfekcji popularnych linii komórkowych przy użyciu odczynników do transfekcji X-tremeGENE™

Protokoły transfekcji popularnych linii komórkowych przy użyciu odczynników do transfekcji X-tremeGENE™

Zobacz wszystko

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