LMDH-RO

L-Malate Dehydrogenase (L-MDH)

from pig heart (mitochondrial)

• Numer EC enzymu: 1.1.1.37 (BRENDA, IUBMB)
• Kod UNSPSC: 12352202

Właściwości

• pochodzenie biologiczne: Porcine heart (mitochondrial)
• Poziom jakości: 100
• Formularz: solution; suspension
• aktywność właściwa: ~1200 units/mg protein (At 25 °C with oxaloacetate as the substrate.)
• opakowanie: pkg of 1 mL (10127248001 solution); pkg of 1 mL (10127256001 suspension); pkg of 5 mL (10127914001suspension)
• producent / nazwa handlowa: Roche
• metody: activity assay: suitable
• kolor: white
• optymalne pH: 7.4-7.5(reduction of oxaloacetate); 9.2-9.5(oxidation of malate)
• rozpuszczalność: water: miscible
• przydatność: suitable for Western blot
• numer dostępu NCBI: NM_001244153.1
• numer dostępu UniProt: P00346
• Zastosowanie: life science and biopharma
• obecność zanieczyszczeń: Fumarase <0.01%; GIDH 0.001%; GOT 0.001%; GPT 0.00185%; LDH <0.01%; NADH oxidase <0.001%
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C
• informacje o genach: Porcine ... MDH2(397039)

Opis

Opis ogólny

L-malate:NAD⁺ oxidoreductase
The L-malate dehydrogenase enzyme is a nuclear gene product that is synthesized with a 24-residue amino-terminal signal peptide. This peptide is proteolytically cleaved during the translocation of the enzyme to the mitochondrial matrix.

Zastosowanie

The enzyme L-malate dehydrogenase from pig heart has been used to measure PEPCK (phosphoenolpyruvate carboxykinase) activity. The oxaloacetate, produced by PEPCK, is reduced to malate via the oxidation of NADH, which in turn is measured at 340 nm using a spectrophotometer. The enzyme has also been used to measure oxaloacetate by measuring the reduction in NADH spectroscopically at 340 nm.

Działania biochem./fizjol.

The enzyme L-malate dehydrogenase from pig heart catalyzes the oxidation of L-malate to oxaloacetate. The enzyme is an NAD-dependent mitochondrial dehydrogenase that functions in the tricarboxylic acid cycle. It is a component of the malate-aspartate shuttle that transports reducing equivalents across the inner mitochondrial membrane in the form of malate.

Jakość

Contaminants: <0.002% GOT, <0.01% fumarase and LDH, each luM each, <0.02% HK, <0.002% PGI

Postać fizyczna

Solution in 50% glycerol (v/v), pH approximately 7

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Uwaga dotycząca przygotowania

Activator: – phosphate
– arsenate
– Mg²⁺
– Zn²⁺

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): No data available
• Temperatura zapłonu (°C): No data available