10128023001

NADH

Grade II, disodium salt

• Synonim(y): β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate, β-DPNH, β-NADH, DPNH, Diphosphopyridine nucleotide, reduced form, NADH
• Wzór empiryczny (zapis Hilla): C₂₁H₂₇N₇Na₂O₁₄P₂ · xH₂O
• Numer CAS: 606-68-8
• Masa cząsteczkowa: 709.40 (anhydrous basis)
• Numer MDL: MFCD03791273
• Kod UNSPSC: 12352204
• Identyfikator substancji w PubChem: 329748955

Właściwości

• Poziom jakości: 100
• Próba: 98%
• Postać: amorphous powder
• masa cząsteczkowa: (NADH-Na₂: M_r = 709.4; NADH: M_r = 665.4)
• opakowanie: pkg of 1 g
• producent / nazwa handlowa: Roche
• warunki przechowywania: protect from light
• λmaks.: 260 and 340 nm
• Warunki transportu: wet ice
• ciąg SMILES: O.[Na+].[Na+].NC(=O)C1=CN(C=CC1)[C@@H]2O[C@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]3O[C@H]([C@H](O)[C@@H]3O)n4cnc5c(N)ncnc45)[C@@H](O)[C@H]2O
• InChI: 1S/C21H29N7O14P2.2Na.H2O/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33;;;/h1,3-4,7-8,10-11,13-16,20-21,29-32H,2,5-6H2,(H2,23,33)(H,34,35)(H,36,37)(H2,22,24,25);;;1H2/q;2*+1;/p-2/t10-,11-,13-,14-,15-,16-,20-,21-;;;/m1.../s1
• Klucz InChI: FWBNCIFELNNJCX-UHOVGGJYSA-L

Opis

Zastosowanie

As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.

NADH has been used in MDH (malate dehydrogenase) enzymatic activity assay.

NADH has been used in lactate dehydrogenase (LDH) enzymatic activity assay, glutamate-cysteine ligase activity assay and fructose 2,6-bisphosphate assay.

Działania biochem./fizjol.

NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, β-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD⁺/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease.
As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.

Opakowanie

Packaged by solid weight.

Jakość

Purity: 98% β-NADH-Na₂ based on dry weight (enzymatically and absorbance at 340 nm), 99% (absorbance at 260 nm), A₂₆₀ nm-A₃₄₀ nm: <2.40
Organic solvents: <4% (GC)

Charakterystyka techniczna

Absorbance coefficients: 14.3 x 10³ l x mol^-1 x cm^-1 at 260 nm, 6.18 x 10³ l x mol^-1 x cm^-1 at 334 nm (Hg), 6.3 x 10³ l x mol^-1 x cm^-1 at 340 nm, 3.4 x 10³ l x mol^-1 x cm^-1 at 365 nm (Hg)

Formula: C₂₁H₂₇N₇O₁₄P₂Na₂

pH value in water: 7.5 ± 0.5 (100 mg/ml)

Postać fizyczna

Amorphous powder

Uwaga dotycząca przygotowania

Activator: Mg²⁺ (5 to 10 mM)
Storage conditions (working solution): 10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADH after 4 weeks at 2 to 8 °C

Przechowywanie i stabilność

Store at 2 to 8 °C. (Store dry!)

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

This page may contain text that has been machine translated.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable