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Merck

11089153001

Roche

Streptavidin-POD conjugate

liquid, pkg of 1 mL

Synonim(y):

streptavidin

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About This Item

Kod UNSPSC:
12352200

Postać

liquid

Poziom jakości

opakowanie

pkg of 1 mL

producent / nazwa handlowa

Roche

temp. przechowywania

-10 to -25°C

Opis ogólny

Streptavidin is covalently coupled to horseradish peroxidase. The conjugate is used for detection of biotin-labelled substances.

Zastosowanie

Streptavidin-POD has been used for:
  • ELISA
  • In situ hybridization
  • Western blot
It is also used for immunohistochemistry.

Definicja jednostki

One unit Streptavidin-POD, corresponding to one unit peroxidase, will oxidize 1 μmol of guaiacol tetramer in one minute at +25 °C and pH 7.0.

Unit Conversion: 1 U (+25 °C; guaiacol as substrate; pH7.0) corresponds to 1.24 U (+25 °C; 1.7 mM ABTS as substrate; pH= 6.0) or to 4.0 U (+25 °C; 8.7 mM ABTS as substrate; pH = 5.0).

Postać fizyczna

Liquid, stabilized

Uwaga dotycząca przygotowania

Working concentration: 5 - 500 mU/ml (i.e., stock solution can be diluted).Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
  • ELISA: 50 to 200 mU/ml
  • Immunohistocytochemistry: 250 to 1000 mU/ml
  • Western blot: 200 to 500 mU/ml

Inne uwagi

For life science research only. Not for use in diagnostic procedures.
This page may contain text that has been machine translated.

Piktogramy

Exclamation mark

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Skin Sens. 1

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

does not flash

Temperatura zapłonu (°C)

does not flash


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Fernanda V Castro et al.
Cancer immunology, immunotherapy : CII, 61(7), 1005-1018 (2011-12-01)
Effective vaccination against tumour-associated antigens (TAA) such as the 5T4 oncofoetal glycoprotein may be limited by the nature of the T cell repertoire and the influence of immunomodulatory factors in particular T regulatory cells (Treg). Here, we identified mouse 5T4-specific
Angelika Hammerer-Lercher et al.
Clinical chemistry, 54(5), 858-865 (2008-03-15)
The specific forms of pro-B-type natriuretic peptide (proBNP) that occur in human blood are not yet clear. We demonstrated the presence of several proBNP forms in human plasma with a new affinity chromatography method that can be used in combination
Triple-Labeling Whole-Mount In Situ Hybridization Method for Analysis of Overlapping Gene Expression in Brain Tissue with High Level of Autofluorescence.
Chen J
Journal of Cytology & Histology, http://dx-http://dx (2015)
Benjamin Meyer et al.
Frontiers in immunology, 13, 841009-841009 (2022-05-20)
To comprehensively evaluate SARS-CoV-2 specific B-cell and antibody responses up to one year after mild COVID-19. In 31 mildly symptomatic COVID-19 participants SARS-CoV-2-specific plasmablasts and antigen-specific memory B cells were measured by ELISpot. Binding antibodies directed against the proteins spike
Husheem Michael et al.
Frontiers in immunology, 11, 196-196 (2020-03-03)
Human rotavirus (HRV) is a leading cause of morbidity and mortality in children, especially in developing countries. Malnutrition is prevalent in these countries, which may contribute to the decreased oral vaccine efficacy, posing a concern for global health. Neonatal gnotobiotic

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