10102547001

AMP-PNP

Adenylyl-imidodiphosphate

• Synonim(y): Adenosine 5′-(β,γ-imido)triphosphate lithium salt hydrate, β,γ-Imidoadenosine 5′-triphosphate lithium salt hydrate, AMP-PNP, ATP[β,γ-NH], Adenylyl imidodiphosphate lithium salt hydrate, App(NH)p
• Wzór empiryczny (zapis Hilla): C₁₀H₁₇N₆O₁₂P₃ · xLi⁺ · yH₂O
• Numer CAS: 25612-73-1
• Masa cząsteczkowa: 506.20 (free acid basis)
• Beilstein: 6047109
• Kod UNSPSC: 12352204

Właściwości

• Postać: powder
• masa cząsteczkowa: M_r 506.2 (ANP-PNP); M_r 529.9 (AMP-PNP-Li₄)
• opakowanie: pkg of 25 mg
• producent / nazwa handlowa: Roche
• ciąg SMILES: [Li+].[Li+].[Li+].[Li+].[H]O[H].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP([O-])(=O)OP([O-])(=O)NP([O-])([O-])=O)[C@@H](O)[C@H]3O
• InChI: 1S/C10H17N6O12P3/c11-8-5-9(13-2-12-8)16(3-14-5)10-7(18)6(17)4(27-10)1-26-31(24,25)28-30(22,23)15-29(19,20)21/h2-4,6-7,10,17-18H,1H2,(H,24,25)(H2,11,12,13)(H4,15,19,20,21,22,23)/t4-,6-,7-,10-/m1/s1
• Klucz InChI: PVKSNHVPLWYQGJ-KQYNXXCUSA-N

Opis

Opis ogólny

AMP-PNP (adenylyl-imidodiphosphate) is a non-hydrolysable analogue of ATP. It inhibits fast axonal transport and facilitates the interaction between membranous organelles and microtubules.

AMP-PNP(adenylyl-imidodiphosphate) is an artificial substrate of enzyme, adenylate cyclase. Adenylate cyclase helps to hydrolyze adenylyl-imidodiphosphate slowly than ATP.

Zastosowanie

AMP-PNP has been used in the preparation of nucleotide solutions, two-motor fluorescence assay, preparation of flow-cells with kinesin and cascade binding assays.

AMP-PNP is a competitive inhibitor of most ATP-dependent systems. A good substrate for enzymes hydrolyzing between the α- and β-phosphorus atom, yet is resistant to enzymes cleaving between the β- and γ-phosphorus atom. Substrate of snake venom phosphodiesterase and adenylate cyclase.
AMP-PNP has been used for two-motor fluorescence assay.

Cechy i korzyści

Contents
88% AMP-PNP (from N), 4.5% lithium, 6% water

Jakość

Contaminants: ≤1.5% Pi , <0.1% ATP (enzymatically)

Przechowywanie i stabilność

Store at -15–-25 °C. (Store dry!)

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable