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SCM075

Sigma-Aldrich

ROCK Inhibitor (Y-27632)

The ROCK Inhibitor (Y-27632) is available in a 5 mg format & has been optimized & validated for cell culture & Neuroscience applications.

Synonim(y):

Y-27632

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About This Item

Kod UNSPSC:
12352207
eCl@ss:
32160801
NACRES:
NA.75

Poziom jakości

Postać

liquid

masa cząsteczkowa

320.26 g/mol

metody

cell culture | mammalian: suitable

Warunki transportu

dry ice

Opis ogólny

ROCK Inhibitor (Y-27632) is a selective, ATP-competitive inhibitor of Rho-associated protein kinase (ROCK) including p160Rock (Ki=140nM) and Rock-II. Also acts as a potent inhibitor of agonist-induced Ca2+ sensitization of myosin phosphorylation and smooth muscle contraction. One-hour treatment with 10uM Y-27632 blocks apoptosis of dissociated cultured human embryonic stem cells (hESCs), increases survival and cloning efficiency of hESCs without affecting their pluripotency1. Recently, some reports showed that the application of Y-27632 and Accutase (Cat. No. SCR005), a formulated mixture of digestive enzymes, enhances the post-thaw survival rate of single hESCs using conventional slow freezing and rapid thawing methods

Przechowywanie i stabilność

Before Reconstitution: Maintain at -20ºC, protected from light for 2 years. After Reconstitution: 1 year at -20ºC, protected from light
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Katarzyna Pańczyk et al.
MedChemComm, 9(11), 1933-1948 (2018-12-21)
A series of 17 new phenoxyacetamides has been prepared via multistep chemical synthesis as a continuation of the research carried out by our group on di- and tri-substituted phenoxyalkyl and phenoxyacetyl derivatives of amines. The obtained compounds vary in an
Maike Frye et al.
eLife, 9 (2020-09-09)
Endothelial integrity is vital for homeostasis and adjusted to tissue demands. Although fluid uptake by lymphatic capillaries is a critical attribute of the lymphatic vasculature, the barrier function of collecting lymphatic vessels is also important by ensuring efficient fluid drainage
Xin Geng et al.
JCI insight, 5(14) (2020-06-17)
During the growth of lymphatic vessels (lymphangiogenesis), lymphatic endothelial cells (LECs) at the growing front sprout by forming filopodia. Those tip cells are not exposed to circulating lymph, as they are not lumenized. In contrast, LECs that trail the growing
Anushree C Gulvady et al.
Molecular biology of the cell, 30(11), 1298-1313 (2019-03-21)
Fibroblasts transformed by the proto-oncogene Src form individual invadopodia that can spontaneously self-organize into large matrix-degrading superstructures called rosettes. However, the mechanisms by which the invadopodia can spatiotemporally reorganize their architecture is not well understood. Here, we show that Hic-5
Xin Lin et al.
EBioMedicine, 69, 103463-103463 (2021-07-06)
Family with Sequence Similarity 13, Member A (FAM13A) gene has been consistently associated with COPD by Genome-wide association studies (GWAS). Our previous study demonstrated that FAM13A was mainly expressed in the lung epithelial progenitors including Club cells and alveolar type

Produkty

Human epithelial intestinal colonic organoids can be used as an alternative to Caco-2 drug permeability assays for drug screening and compound toxicity testing.

Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.

Organoidy płuc są cennymi modelami 3D dla rozwoju ludzkich płuc i chorób układu oddechowego. Protokół różnicowania 3dGRO™ generuje organoidy z ludzkich iPSC w 4 krokach.

Zapoznaj się z rodzajami komórek macierzystych, w tym multipotencjalnymi komórkami macierzystymi, pluripotencjalnymi komórkami macierzystymi i iPSC oraz ich zastosowaniami w podstawowych badaniach nad komórkami macierzystymi, terapii komórkami macierzystymi i modelowaniu chorób.

Zobacz wszystko

Protokoły

A rapid in vitro assay for CFTR function, the forskolin-induced swelling protocol uses human colon organoids, which can be derived from cystic fibrosis patient tissue.

Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.

Protokoły hodowli ludzkich indukowanych pluripotencjalnych komórek macierzystych (iPSC) krok po kroku, w tym rozmrażanie, ekspansja, zamrażanie i charakteryzowanie komórek ips.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej