420220-M

Thiazovivin

≥98% (HPLC), powder, Rho kinase inhibitor, Calbiochem

• Synonim(y): iPSC Induction Enhancer, Thiazovivin, N-Benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide, Induced Pluripotent Stem Cells Induction Enhancer, Rho Kinase Inhibitor XII
• Wzór empiryczny (zapis Hilla): C₁₅H₁₃N₅OS
• Masa cząsteczkowa: 311.36
• Kod UNSPSC: 12352200

Właściwości

• Nazwa produktu: iPSC Induction Enhancer, Thiazovivin, A cell-permeable aminothiazolo-carboxamide compound that is reported to augment SB431542 and PD0325901.
• Poziom jakości: 100
• Próba: ≥98% (HPLC)
• Formularz: powder
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; protect from light
• kolor: tan
• rozpuszczalność: DMSO: 100 mg/mL
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

A cell-permeable aminothiazolo-carboxamide compound that is ~5-fold more potent than Y-27632 (Cat. Nos. 688000 & 688001) in inhibiting ROCK enzymatic activity (71% inhibition at 2 µM). Single-cell dissociation of cultured hESCs is demonstrated to hyperactivate Rho-ROCK signaling and result in cell death, while suppression of such hyperactivation by Tzv (2 µM) or Y-27632 (2 µM) stabilize newly synthesized cell surface E-cadherin, which in turn promotes cell-cell adhesion and prevents trypsinization-induced apoptosis (12% vs 81% TUNEL-positive cells after trypsinization in matrigel cultures with 2 µM Tzv or DMSO, respectively). Unlike, Ptn (Cat. No. 544049), Tzv protects dissociated individual hESCs even in the absence of ECM (24% vs 90% TUNEL-positive cells after trypsinization in non-coating plates with 2 µM Tzv or Ptn, respectively). Simultaneous blockage of TGFβ and MEK-ERK pathways by 2 µM SB431542 (Cat. No. 616461)and 0.5 µM PD0325901 (Cat. No. 444966) is reported to result in >50-fold improvement of human iPSCs (induced Pluripotent Stem Cells) generation from fibroblast cultures virally transfected with 4-TF (Oct44, Klf4, Sox2, and c-Myc), while the inclusion of 0.5 µM Tzv on top of SB431542 and PD0325901 is demonstrated to further improve the efficiency to an overall improvement of >200-fold from 4-TF-induction alone.

Ostrzeżenie

Toxicity: Standard Handling (A)

Rekonstytucja

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Inne uwagi

Xu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA107, 8129
Lin, T., et al. 2009. Nat. Methods6, 805.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable