MABT880

Anti-SUN2 Antibody, clone 3.1E

clone 3.1E, from mouse

• Synonim(y): SUN domain-containing protein 2, Protein unc-84 homolog B, Rab5-interacting protein, Rab5IP, Sad1/unc-84 protein-like 2
• Kod UNSPSC: 12352203
• eCl@ss: 32160702

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: 3.1E, monoclonal
• reaktywność gatunkowa: human, rat, mouse
• metody: immunocytochemistry: suitable; western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NP_001186509.1
• numer dostępu UniProt: Q9UH99
• Warunki transportu: ambient
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... SUN2(25777)

Opis

Opis ogólny

SUN domain-containing protein 2 (UniProt Q9UH99; also known as Protein unc-84 homolog B, Rab5-interacting protein, Rab5IP, Sad1/unc-84 protein-like 2) is encoded by the SUN2 (also known as FRIGG, KIAA0668, RAB5IP, UNC84B) gene (Gene ID 25777) in human. It is a single-pass nuclear envelope transmembrane protein that is highly expressed in heart, lung, and muscle. It has a predicted transmembrane domain and a C-terminal region with similarity to the S. pombe spindle pole body protein Sad1. It may also facilitate a nuclear-centrosomal interaction required for nuclear migration and anchorage. It anchors chromosome movement in the prophase of meiosis and is required for telomere attachment to nuclear envelope and gametogenesis. SUN2 protein may also function on endocytic vesicles as a receptor for RAB5-GDP and participate in the activation of RAB5. Slight overexpression of SUN2 protein is seen in heart tissues form patients with congenital heart defects.

Specyficzność

Target specificity of clone 3.1E was verified by immunocytochemistry and Western blotting analyses using fibroblasts and heart tissue samples from Sun2-knockout mice.

Immunogen

GST-tagged recombinant human SUN2 N-terminal LMNA-binding region fragment.

Zastosowanie

Research Category
Cell Structure

This mouse monoclonal Anti-SUN2 Antibody, clone 3.1E, Cat. No. MABT880, is validated for use in Immunocytochemistry and Western Blotting for the detection of SUN2.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected SUN2 in 10 µg of HepG2 cell lysate.

Immunocytochemistry Analysis: A representative lot immunostained nucleus of fibroblasts from Sun2+/-, but not Sun2-/- mice (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).

Western Blotting Analysis: A representative lot detected SUN2 in heart tissue lysate from Sun2+/-, but not Sun2-/- mice (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).

Immunofluorescence Analysis (IF): A representative lot detected mouse testis tissue (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).

Immunofluorescence Analysis (IF): A representative lot tested on different mouse tissues (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).

Immunofluorescence Analysis (IF): A representative lot detected immortalized fibroblasts derived from SUN2 +/+, SUN2 -/- , Sun1 -/- double knockout Sun2 -/- Sun1 -/- mouse pups (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).

Jakość

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SUN2 in 10 µg of HeLa cell lysate.

Opis wartości docelowych

~80 kDa observed. 80.31/82.50/79.61 kDa (isoform 1/2/3) calculated. Uncharacterized bands may be observed in some lysate(s).

Postać fizyczna

Format: Purified

Protein G purified.

Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1