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12103C

Sigma-Aldrich

Fetal Bovine Serum

USA origin, suitable for cell culture

Synonim(y):

Fetal Calf Sera, Fetal Calf Serum, FBS, FCS, sera, serum

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About This Item

Numer MDL:
MFCD00132239
Kod UNSPSC:
12352207
NACRES:
NA.71

pochodzenie biologiczne

bovine fetus

Poziom jakości

500

linia produktu

FBS Premier

skład

hemoglobin, ≤20 mg/dL

pochodzenie

USA origin

metody

cell culture | mammalian: suitable

zanieczyszczenia

≤10 EU/mL endotoxin

Warunki transportu

dry ice

temp. przechowywania

−20°C

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Opis ogólny

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Zastosowanie

Fetal Bovine Serum was used in different types of cell culture as a supplement, such as the following:
  • PC12 cells grown in Dulbecco′s modified Eagle′s medium supplemented with Fetal Bovine Serum along with other components.
  • HeLa cells were maintained in Roswell Park Memorial Institute (RPMI) 1640 medium containing Fetal Bovine Serum along with other components.
  • BaF3 Cell Culture (cells were maintained in RPMI 1640 medium supplemented with Fetal Bovine Serum along with other components).
  • Primary hippocampal cell cultures (cells were maintained in Eagle′s minimum essential medium and supplemented with heat-inactivated fetal bovine serum along with other components).
  • HeLa, Cos1 and Cos7 cells were cultured in Dulbecco modified Eagle medium (DMEM) with fetal bovine serum along with other components.
Fetal Bovine Serum (FBS) is the most common serum used to supplement cell culture media due to its high nutritional content. Although it is relatively low in protein, FBS is effective in promoting and sustaining growth of vertebrate mammalian and insect cells.

This product is for further manufacturing use. THIS PRODUCT IS NOT INTENDED FOR HUMAN OR THERAPEUTIC USE.

Komentarz do analizy

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

R J Mark et al.
Journal of neurochemistry, 68(1), 255-264 (1997-01-01)
Peroxidation of membrane lipids results in release of the aldehyde 4-hydroxynonenal (HNE), which is known to conjugate to specific amino acids of proteins and may alter their function. Because accumulating data indicate that free radicals mediate injury and death of
Joseph E Rabinowitz et al.
Journal of virology, 76(2), 791-801 (2001-12-26)
The serotypes of adeno-associated virus (AAV) have the potential to become important resources for clinical gene therapy. In an effort to compare the role of serotype-specific virion shells on vector transduction, we cloned each of the serotype capsid coding domains
Julie L Webb et al.
The Journal of biological chemistry, 278(27), 25009-25013 (2003-04-30)
Parkinson's disease (PD) is characterized by the loss of dopaminergic neurons in the substantia nigra and the formation of aggregates (Lewy bodies) in neurons. alpha-Synuclein is the major protein in Lewy bodies and rare mutations in alpha-synuclein cause early-onset PD.
Maria C Cuitiño et al.
Cell reports, 27(12), 3547-3560 (2019-05-28)
Orchestrating cell-cycle-dependent mRNA oscillations is critical to cell proliferation in multicellular organisms. Even though our understanding of cell-cycle-regulated transcription has improved significantly over the last three decades, the mechanisms remain untested in vivo. Unbiased transcriptomic profiling of G0, G1-S, and S-G2-M
Tadepally Lakshmikanth et al.
Cell reports, 32(3), 107923-107923 (2020-07-23)
The human immune system varies extensively between individuals, but variation within individuals over time has not been well characterized. Systems-level analyses allow for simultaneous quantification of many interacting immune system components and the inference of global regulatory principles. Here, we
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Protokoły

Zamrażanie i rozmrażanie FBS i innych surowic

Chroń swoją inwestycję dzięki najlepszym praktykom przechowywania i rozmrażania FBS i innych surowic. Próbki FBS do walidacji w protokole hodowli komórkowej.

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