MABT535
Anti-Desmin Antibody, clone 4C12.1
clone 4C12.1, from mouse
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.43
klon:
4C12.1, monoclonal
application:
IHC
WB
WB
reaktywność gatunkowa:
mouse, rat, human
metody:
immunohistochemistry: suitable (paraffin)
western blot: suitable
western blot: suitable
citations:
1
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
4C12.1, monoclonal
reaktywność gatunkowa
mouse, rat, human
opakowanie
antibody small pack of 25 μg
metody
immunohistochemistry: suitable (paraffin)
western blot: suitable
izotyp
IgG2bκ
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
ambient
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... DES(1674)
Opis ogólny
Desmin (UniProt: P17661) is encoded by the DES gene (Gene ID: 1674) in human. Desmin is a major class III intermediate filament protein essential for the structural integrity and function of muscle.
In adult striated muscle desmin forms a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. It may also act as a sarcomeric microtubule-anchoring protein and specifically associate with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Desmin is particularly abundant at the myotendinous junctions and at the neuromuscular junctions of skeletal muscle. Mammalian cardiac muscle cells contain higher amounts of desmin (up to 2% of total protein) compared to skeletal muscle cells (0.35% of total protein). Mutations in DES gene have been linked to familial cardiac and skeletal myopathy characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, and restrictive heart failure. It can also lead to myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells. (rEF.: Denise Paulin, D., and Li, Z (2004). Experimental Cell Research 301(1); 1 -7).
In adult striated muscle desmin forms a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. It may also act as a sarcomeric microtubule-anchoring protein and specifically associate with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Desmin is particularly abundant at the myotendinous junctions and at the neuromuscular junctions of skeletal muscle. Mammalian cardiac muscle cells contain higher amounts of desmin (up to 2% of total protein) compared to skeletal muscle cells (0.35% of total protein). Mutations in DES gene have been linked to familial cardiac and skeletal myopathy characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, and restrictive heart failure. It can also lead to myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells. (rEF.: Denise Paulin, D., and Li, Z (2004). Experimental Cell Research 301(1); 1 -7).
Specyficzność
Clone 4C12.1 specifically detects Desmin in human, mouse, and rat muscles. It targets an epitope within 64 amino acids from the C-terminal end.
Immunogen
GST/His-taged recombinant fragment corresponding to 64 amino acids from the C-terminal region of human Desmin.
Zastosowanie
Anti-Desmin Antibody, clone 4C12.1, Cat. No. MABT535, is a mouse monoclonal antibody that targets Desmin in Human, Mouse, and Rat and it is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Desmin in mouse heart, mouse skeletal muscle, rat heart, and rat skeletal muscle tissues.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Desmin in human heart and human skeletal muscle tissues.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Desmin in human heart and human skeletal muscle tissues.
Research Category
Cell Structure
Cell Structure
Jakość
Evaluated by Western Blotting in human skeletal muscle tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Desmin in 10 µg of human skeletal muscle tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Desmin in 10 µg of human skeletal muscle tissue lysate.
Opis wartości docelowych
~53 kDa observed; 53.53 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Postać fizyczna
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Inne uwagi
Concentration: Please refer to lot specific datasheet.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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