MABT121

Anti-Vimentin Antibody, clone 3CB2

clone 3CB2, from mouse

• Synonim(y): Vimentin
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• klon: 3CB2, monoclonal
• application: ICC; IHC; WB
• reaktywność gatunkowa: chameleon (GLIA 15:389-400 (1995), Prada, F. A., et. al.), rat (GLIA 15:389-400 (1995), Prada, F. A., et. al.), human, lizard
• metody: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• citations: 3

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: 3CB2, monoclonal
• reaktywność gatunkowa: chameleon (GLIA 15:389-400 (1995), Prada, F. A., et. al.), rat (GLIA 15:389-400 (1995), Prada, F. A., et. al.), human, lizard
• reaktywność gatunkowa (przewidywana na podstawie homologii): chicken (immunogen homology)
• metody: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• izotyp: IgMκ
• numer dostępu NCBI: NP_001041541
• numer dostępu UniProt: P09654
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... VIM(7431)

Opis

Opis ogólny

Vimentin is a class III intermediate filament protein (homopolymers) found in various non-epithelial cells including mesenchymal cells and fibroblasts. In many cells of mesenchymal origin, it is one of the most prominent phosphoproteins, especially during cell division when enhanced phosphorylation of vimentin corresponds with the reorganization of vimentin filaments.

Immunogen

Vimentin extracted from chicken embryonic brain tissue.

Zastosowanie

Anti-Vimentin Antibody, clone 3CB2 is an antibody against Vimentin for use in WB, IC, IH.

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in human central and peripheral retinal sections (Perez-Alvarez, M.J., et al. (2008). J Neurosci Res. 86(8):1871-1883).

Western Blot Analysis:
A representative lot was used to detect vimentin in chicken and rat total brain extracts (Prada, FA., et al. (1995). GLIA 15:389-400).

Immunohistochemistry Analysis:
A representative lot was used to detect vimentin in E3 chick embryo sections and different regions of the developing chick central nervous system (Prada, FA., et al. (1995). GLIA 15:389-400).

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Jakość

Evaluated by Western Blot in ARPE19 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Vimentin in ARPE19 cell lysate.

Opis wartości docelowych

~54 kDa observed

Postać fizyczna

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
ARPE cell lysate

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable