MABN391

Anti-FIS1 Antibody, Clone 4D1.1

clone 4D1.1, from mouse

• Synonim(y): FIS-1, FIS 1, FIS1 homolog, Mitochondrial fission 1 protein, hFis1, hFis-1hFis 1, Tetratricopeptide repeat protein 11, Short name=TPR repeat protein 11
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 4D1.1, monoclonal
• reaktywność gatunkowa: human, rat
• metody: immunohistochemistry: suitable (paraffin); western blot: suitable
• izotyp: IgG2bκ
• numer dostępu NCBI: NP_057152
• numer dostępu UniProt: Q9Y3D6
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... FIS1(51024)

Opis

Opis ogólny

FIS1 or TTC11, or FIS1 homolog (hFis1), or Tetratricopeptide repeat protein 11 (TPR repeat protein 11) or Mitochondrial fission 1 protein is an interesting member of mitochondrial essential proteins. FIS1 is encoded by the nuclear gene FIS1 and is a key player in mitochondrial dynamics. In eukaryotic cells, the shape of mitochondria can be tuned to various physiological conditions by a balance of fusion and fission processes termed mitochondrial dynamics. Mitochondrial dynamics control not only the morphology but also the function of mitochondria, and therefore is crucial in many aspects of a cell′s life. Thus there is a dynamic balance between fission and fusion events that can promote health and normal cell growth or when dysfunctional mitochondrial dynamics have been implicated in a variety of human diseases including cancer. Human FIS1 a mitochondrial, outer membrane localized, adaptor protein that is critical to recruiting the cellular machinery needed for mitochondrial fission or fusion. FIS1 is inserted into the mitochondrial outer membrane via a C-terminal transmembrane domain that, along with a short basic segment, is essential for its targeting to the mitochondria. Overexpression of FIS1 in human cells causes mitochondrial fragmentation and aggregation. FIS1 interacts with the dynamin-related GTPase DLP1/DRP1, and along with other adaptor proteins such as MDV1, MFF, and MiD, the dynamin-related proteins form spirals around constricted sites on mitochondria and assist in the fission of it. In addition to mitochondria, FIS1 also mediates peroxisome fission via the interaction with DLP1 as well. EMD-Millipore’s Anti-FIS1 rabbit polyclonal antibody has been tested in western blot on HeLa cell extracts and in immunohistochemistry on human brain tissue sections.

Immunogen

GST-tagged recombinant protein corresponding to Human FIS1.

Zastosowanie

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected FIS1 in human and rat cerebral cortex tissue.

Research Category
Neuroscience

Neuroscience

Research Sub Category
Neurodegenerative Diseases

Apoptosis - Additional

This Anti-FIS1 Antibody, Clone 4D1.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of FIS1.

Jakość

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected FIS1 in 10 µg of Raji cell lysate.

Opis wartości docelowych

~ 17 kDa observed

Postać fizyczna

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable