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MABN2397

Sigma-Aldrich

Anti-MYCBP2 Antibody, clone PM-8A9

clone PM-8A9, from mouse

Synonim(y):

E3 ubiquitin-protein ligase MYCBP2, Myc-binding protein 2, Pam/highwire/rpm-1 protein, Protein associated with Myc

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified antibody

rodzaj przeciwciała

primary antibodies

klon

PM-8A9, monoclonal

reaktywność gatunkowa

human

metody

immunoprecipitation (IP): suitable
western blot: suitable

izotyp

IgG2aκ

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

ambient

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... MYCBP2(23077)

Opis ogólny

E3 ubiquitin-protein ligase MYCBP2 (UniProt: O75592; also known as Myc-binding protein 2, Pam/highwire/rpm-1 protein, Protein associated with Myc, MYCBP2) is encoded by the MYCBP2 (also known as KIAA0916, PAM) gene (Gene ID: 23077) in human.MYCBP2 protein mediates the ubiquitination and subsequent proteasomal degradation of TSC2/tuberin. It is ubiquitously expressed, but brain and thymus display exceptionally abundant expression. It is shown to co-localize with TSC1 and TSC2 along the neurites and in the growth cones. MYCBP2 may also function as a facilitator or regulator of transcriptional activation by MYC and have a role during synaptogenesis. It is reported to negatively regulate neuronal growth, synaptogenesis, and synaptic strength. Mice with MYCBP2-deficiency in peripheral sensory neurons show prolonged thermal hyperalgesia. Loss of MYCBP2 is also linked to constitutively activated p38 MAPK and increased expression of several proteins involved in receptor trafficking. Down-regulation of MYCBP2 in the spinal cord of adult rats is shown to enhance their. MYCBP2 is also shown to be an inhibitor of adenylyl cyclase. (Ref.: Holland, S et al. (2011). J. Biol. Chem. 286(5): 3671-3680).

Specyficzność

Clone PM-8A9 specifically detects MYCBP2 in HEK293 cells. It targets an epitope within the first 150 amino acids in the N-terminal region.

Immunogen

Epitope: N-terminus
MBP (Maltose-binding protein)-tagged recombinat fragment of 150 amino acids from the N-terminal region of human MYCBP2.

Zastosowanie

Anti-MYCBP2 Antibody, clone PM-8A9, Cat. No. MABN2397, is a highly specific mouse monoclonal antibody that targets MYCBP2 (PAM) and has been tested in Immunoprecipitation and Western blotting.
Immunoprecipitation Analysis: A representative lot detected MYCBP2 (Courtesy of Roberta L. Beauchamp, Massachusetts General Hospital, Boston, USA).

Western Blotting Analysis: A representative lot detected endogenous PAM (MYCBP2)and overexpressed Myc-tagged PAM (Courtesy of Roberta L. Beauchamp, Massachusetts General Hospital, Boston, USA).
Research Category
Neuroscience

Jakość

Evaluated by Western Blotting in HEK293 cell lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected MYCBP2 in 10 µg of HEK293 cell lysate.

Opis wartości docelowych

~460 kDa observed; 510.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Postać fizyczna

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1


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Odwiedź Bibliotekę dokumentów

Gongsheng Yuan et al.
International journal of oral science, 15(1), 11-11 (2023-02-17)
Tumor-associated macrophages (TAMs) play crucial roles in tumor progression and immune responses. However, mechanisms of driving TAMs to antitumor function remain unknown. Here, transcriptome profiling analysis of human oral cancer tissues indicated that regulator of G protein signaling 12 (RGS12)

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