Przejdź do zawartości
Merck
Wszystkie zdjęcia(1)

Key Documents

MABN1794

Sigma-Aldrich

Anti-GnRH-R Antibody, clone F1G4

clone FIG4, from mouse

Synonim(y):

Gonadotropin-releasing hormone receptor, GnRH receptor, GnRH-R, Leutinizing hormone-releasing hormone receptor, Luteinizing-releasing hormone receptor

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

FIG4, monoclonal

reaktywność gatunkowa

human

metody

ELISA: suitable
dot blot: suitable
flow cytometry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

izotyp

IgG1λ

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... GNRHR (2798)

Opis ogólny

Gonadotropin-releasing hormone receptor (UniProt P30968; also known as GnRH receptor, GnRH-R, Leutinizing hormone-releasing hormone receptor, Luteinizing-releasing hormone receptor) is encoded by the GNRHR (also known as GNRHR1, GRHR, LHRHR, LRHR) gene (Gene ID 2798) in human. GnRH-R belongs to the family of seven-transmembrane G-protein coupled receptors (GPCRs). GnRH-R resides primarily in the pituitary and is responsible for eliciting the actions of GnRH (also known as Luteinizing hormone-releasing hormone or LHRH) after its release from the hypothalamus. Luteinizing hormone (LH) is then secreted from gonadotropes of the anterior pituitary into the bloodstream following pulsatile stimulation of GnRH-R by GnRH/LHRH. The canonical GnRH-R designated by UniProt (P30968-1) corresponds to a 328-amino acid sequence that includes 4 extracellular domains (a.a. 1-38, 98-115, 185-212, 233-281), 7 transmembrane segments (a.a. 39-58, 78-97, 116-137, 165-184, 213-232, 282-300, 307-326), 4 intracellular domains (a.a. 59-77, 138-164, 233-281, 327-328). Multiple GnRH-R variants and post-translationally processed forms are reported in human brain samples.

Specyficzność

Specificity of clone F1G4 was demonstrated by tissue-specific staining and by immunogen peptide blocking (Karande, A.A., et al. (1995). Mol. Cell. Endocrinol. 114(102):51-56).

Immunogen

BSA-conjugated linear peptide corresponding to the N-terminal sequence of human GnRH-R.
Epitope: N-terminal extracellular domain.

Zastosowanie

Detect GnRH-R using this Anti-GnRH-R Antibody, clone F1G4 validated for use in Western Blotting, Dot Blot, ELISA, Flow Cytometry, Immunohistochemistry (Paraffin).
Dot Blot Analysis: A representative lot detected the immunogen peptide by dot blot (Karande, A.A., et al. (1995). Mol. Cell. Endocrinol. 114(102):51-56).
ELISA Analysis: A representative lot detected the immobilized immunogen peptide by ELISA (Karande, A.A., et al. (1995). Mol. Cell. Endocrinol. 114(102):51-56).
Flow Cytometry Analysis: A representative lot detected surface GnRH-R immunoreactivity on live human breast carcinoma T47D and ovarian carcinoma OVCAR-3 cells. Most likely due to low affinity antibody binding or unoptimized antibody concentration used, only ~50% of the T47D and ~10% of the OVCAR-3 populations were stained (Karande, A.A., et al. (1995). Mol. Cell. Endocrinol. 114(102):51-56).
Immunohistochemistry Analysis: A rerepsentative lot detected GnRH-R expression on gonadotropin-producing endocrine cells (gonadotropes) in pituitary, as well as among neurons in human hippocampus and neocortex, using formalin-fixed, paraffin-embedded human tissue sections (Wilson, A.C., et al. (2006). J. Endocrinol. 191(3):651–663).
Immunohistochemistry Analysis: A rerepsentative lot detected similar hippocampus GnRH-R immunoreactivity among Alzheimer′s diseased (AD) and age-matched control brain sections, while significanly decreased GnRH-R immunoreactivity associated with the apical dendrites of pyramidal neurons was seen in the AD brain using formalin-fixed, paraffin-embedded tissue sections (Wilson, A.C., et al. (2006). J. Endocrinol. 191(3):651–663).
Immunohistochemistry Analysis: A rerepsentative lot detected GnRH-R-positive cells in the anterior pituitary, but not the posterior pituitary using frozen human brain sections. Pre-blocking with immunogen peptide abolished the antibody staining (Karande, A.A., et al. (1995). Mol. Cell. Endocrinol. 114(102):51-56).
Western Blotting Analysis: A rerepsentative lot detected the expression of multiple GnRH-R variants in human cortex and pituitary tissue lysates, including the most prominent 30 kDa, 64 kDa, and 136 kDa bands. The 136 kDa band was seen significantly downregulated in the cortex, but not pituitary from Alzheimer′s diseased (AD) brain when compared to age-matched control (Wilson, A.C., et al. (2006). J. Endocrinol. 191(3):651–663).
Research Category
Neuroscience
Research Sub Category
Hormones & Receptors

Jakość

Evaluated by Western Blotting in human pituitary tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected GnRH-R in 10 µg of human pituitary tissue lysate.

Opis wartości docelowych

~34 kDa observed. Clone F1G4 was reported to detect multiple GnRH-R variants and posttranslationally processed forms in human brain samples (Wilson, A.C., et al. (2006). J. Endocrinol. 191(3):651–663).

Postać fizyczna

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1λ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This page may contain text that has been machine translated.

Nie możesz znaleźć właściwego produktu?  

Wypróbuj nasz Narzędzie selektora produktów.

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej