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MABN15

Sigma-Aldrich

Anti-Rhodopsin Antibody, clone 4D2

clone 4D2, from mouse

Synonim(y):

rhodopsin, rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant), retinitis pigmentosa 4, autosomal dominant, opsin 2, rod pigment

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

4D2, monoclonal

reaktywność gatunkowa

rat, human, fish

reaktywność gatunkowa (przewidywana na podstawie homologii)

mouse (based on 100% sequence homology)

metody

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

izotyp

IgG2bκ

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... RHO(6010)
rat ... Rho(24717)

Opis ogólny

Rhodopsin is a multi-pass, G-protein-coupled membrane receptor protein belonging to the G-protein coupled receptor 1 family. Found within rod photoreceptor cells, rhodopsin is activated by light photons in order for the visual transduction cascade to initialize. Rhodopsin is necessary to the forming of images in low light and for the viability of photoreceptor cells after birth. Mutations in rhodopsin expression are the most widely known cause of the human disease retinitis pigmentosa (RP) resulting in gradual loss of vision. At present, there are over 100 known rhodopsin mutations.

Specyficzność

This antibody recognizes Rhodopsin at the N-terminus.

Immunogen

Epitope: N-terminus
Rat rod outer segments corresponding to rat Rhodopsin at the N-terminus.

Zastosowanie

Anti-Rhodopsin Antibody, clone 4D2 is an antibody against Rhodopsin for use in IH, WB, IC, ELISA.
Research Category
Neuroscience
Research Sub Category
Sensory & PNS
Western Blot Analysis: A previous lot of this antibody detected Rhodopsin by WB in goldfish retina lysate, as reported by Knight K, et al.

Immunocytochemistry: A previous lot of this antibody detected Rhodopsin by ICC in goldfish retina tissue, as reported by Knight K, et al

Immunohistochemistry Analysis: 1:500 dilution of this antibody has been shown to detect Rhodopsin in human eye tissue.

ELISA: Specificity of this antibody, from a different lot, was validated in a competitive ELISA, as reported by Laird D, et al.

Jakość

Evaluated by Immunohistochemistry in rat eye tissue.

Immunohistochemistry Analysis: 1:500 dilution of this antibody detected Rhodopsin in rat eye tissue.

Opis wartości docelowych

Expected at ~ 39 kDa

Postać fizyczna

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
Rat eye tissue

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Zhe Zhang et al.
The Journal of biological chemistry, 295(34), 12153-12166 (2020-07-09)
Pheromones play critical roles in habitat identification and reproductive behavior synchronization in the sea lamprey (Petromyzon marinus). The bile acid 3-keto petromyzonol sulfate (3kPZS) is a major component of the sex pheromone mixture from male sea lamprey that induces specific
Liang Jia et al.
The Journal of biological chemistry, 297(5), 101268-101268 (2021-10-04)
Biogenic amines activate G-protein-coupled receptors (GPCRs) in the central nervous system in vertebrate animals. Several biogenic amines, when excreted, stimulate trace amine-associated receptors (TAARs), a group of GPCRs in the main olfactory epithelium, and elicit innate behaviors. How TAARs recognize
The Effect of Transient Local Anti-inflammatory Treatment on the Survival of Pig Retinal Progenitor Cell Allotransplants.
Abud, M; Baranov, P; Hicks, C; Patel, S; Lieppman, B; Regatieri, C; Sinden, J; Isaac et al.
Translational vision science & technology null
Alireza Badiei et al.
Experimental eye research, 181, 72-84 (2019-01-18)
Chronic exposure of the retina to light and high concentrations of polyunsaturated fatty acid in photoreceptor cells make this tissue susceptible to oxidative damage. As retinal degenerative diseases are associated with photoreceptor degeneration, the antioxidant activity of both hydrogen sulfide
Roni A Hazim et al.
Experimental eye research, 179, 18-24 (2018-10-20)
Human RPE cell lines, especially the ARPE-19 cell line, are widely-used in eye research, as well as general epithelial cell studies. In comparison with primary RPE cells, they offer relative convenience and consistency, but cultures derived from these lines are typically

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