MABF850

Anti-IDO-1 Antibody, clone 4B7 | MABF850

clone 4B7, from mouse

• Synonim(y): Indoleamine 2,3-dioxygenase 1, IDO-1, Indoleamine-pyrrole 2,3-dioxygenase
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 4B7, monoclonal
• reaktywność gatunkowa: mouse, rat
• metody: immunohistochemistry: suitable (paraffin); western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NP_032350
• numer dostępu UniProt: P28776
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: mouse ... Ido1(15930)

Opis

Opis ogólny

Indoleamine 2,3-dioxygenase 1 (EC 1.13.11.52; UniProt P28776; also known as IDO-1, indole 2,3-dioxygenase, Indoleamine-pyrrole 2,3-dioxygenase) is encoded by the Ido1 (also known as Ido, Indo) gene (Gene ID 15930) in murine species. Indoleamine 2,3-dioxygenase-1 (IDO1) is a heme containing enzyme that catalyzes the rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. The reduction in local tryptophan and the upregulated immunomodulatory tryptophan metabolites form the basis of IDO-dependent regulation of inflammation and immunity. IDO is reported to support carcinogenesis by modulating tissue microenvironment inflammatory responses. IDO is also reported to mediate immune escape in cancer by suppressing the function of T effector cells and promote the differentiation of T regulatory cells.

Specyficzność

Target specificity of clone 4B7 has been confirmed by both immunohistochemistry and Western blotting using tissue samples from wild-type and Ido1-knockout mice (Thomas, S., et al. (2014). J. Cell. Biochem. 115(2):391-396).

Immunogen

KLH-conjugated linear peptide corresponding to the N-terminal region of mouse IDO-1.

Zastosowanie

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected IDO-1 in rat kidney tissue.
Immunohistochemistry Analysis: A representative lot detected IDO-1 immunoreactivity in acetone-fixed frozen colon tissue sections from wild-type, but not Ido1-knockout mice (Courtesy of Dr. Sunil Thomas, Lankenau Institute for Medical Research, Wynnewood, PA).
Immunofluorescence Analysis: A representative lot detected IDO-1 immunoreactivity in acetone-fixed frozen colon and epididymis tissue sections from wild-type, but not Ido1-knockout mice by fluorescent immunohistochemistry (Courtesy of Dr. Sunil Thomas, Lankenau Institute for Medical Research, Wynnewood, PA).
Western Blotting Analysis: A representative lot detected IDO-1 in epididymis and colon tissue extracts from wild-type, but not Ido1-knockout mice (Thomas, S., et al. (2014). J. Cell. Biochem. 115(2):391-396).
Immunohistochemistry Analysis: A representative lot detected IDO-1 immunoreactivity in multiple acetone-fixed frozen tissue sections from wild-type, but not Ido1-knockout mice, including epididymis, colon, heart, liver, and 4T1-metastasized lung tissue sections (Thomas, S., et al. (2014). J. Cell. Biochem. 115(2):391-396).

This Anti-IDO-1 Antibody, clone 4B7 | MABF850 is validated for use in Immunohistochemistry (Paraffin), Western Blotting for the detection of IDO-1.

Jakość

Evaluated by Immunohistochemistry in mouse kidney tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected IDO-1 in mouse kidney tissue.

Opis wartości docelowych

~45 kDa calculated

Postać fizyczna

Format: Purified

Inne uwagi

Concentration: Please refer to lot specific datasheet.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable