MABE56

Anti-pan Ago Antibody, clone 2A8

clone 2A8, from mouse

• Synonim(y): eukaryotic translation initiation factor 2C, 2, protein argonaute-2, argonaute 2, Protein slicer, argonaute2, PAZ Piwi domain protein, Eukaryotic translation initiation factor 2C, Argonaute2, Argonaute4, Ago4, Protein argonaute-4
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 2A8, monoclonal
• reaktywność gatunkowa: human
• opakowanie: antibody small pack of 25 μg
• metody: immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NP_001158095
• numer dostępu UniProt: Q9UKV8
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... AGO1(26523)

Opis

Opis ogólny

Argonaute (Ago) proteins are a family of proteins of ~95 kDa that bind directly to mature miRNAs. In mammals, there are four Ago proteins (Ago1-4). This family of proteins is a central component of most mammalian miRNPs that mediates important functions in small RNA-directed regulatory pathways. The Ago family consists of two subclases, Ago and Piwi containing two characteristic domains known as PAZ and PIWI. These two domains interact with different parts of the mature miRNA. The PAZ domain interacts with the 3’-end of the miRNA, and the PIWI domain interacts with the 5’ phosphate and 5’-proximal region of the miRNA to guide target mRNA recognition.

Immunogen

Histidine - tagged recombinant protein corresponding to human Ago.

Zastosowanie

Anti-pan Ago Antibody, clone 2A8 is a Mouse Monoclonal Antibody for detection of pan Ago also known as protein argonaute-2 or PAZ Piwi domain protein & has been validated in WB, IP, ICC & IHC.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Nelson, P., et al. (2007). RNA. 13:1787–1792.)

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Nelson, P., et al. (2007). RNA. 13:1787–1792.)

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Nelson, P., et al. (2007). RNA. 13:1787–1792.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

RNA Metabolism & Binding Proteins

Jakość

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Ago on 10 µg of HeLa cell lysate.

Opis wartości docelowych

~ 95 and 70 kDa observed. This antibody recognizes Ago1, Ago2, Ago3, and Ago4.
Note that this antibody has been reported to cross-react with radixin (~70 kDa). (Nelson, P., et al. (2007). RNA. 13:1787–1792.)

Postać fizyczna

Format: Purified

Protein G

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
HeLa cell lysate

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable