MABE1116

Anti-DNAJB1/Hdj1 Antibody, clone J25

clone J25, from mouse

• Synonim(y): Hdj1, DnaJ homolog subfamily B member 1, DNAJB1, hDj-1, Heat shock 40 kDa protein 1, Heat shock protein 40, HSP40
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: J25, monoclonal
• reaktywność gatunkowa: rat, mouse, human
• reaktywność gatunkowa (przewidywana na podstawie homologii): bovine (based on 100% sequence homology)
• metody: flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• izotyp: IgG2bκ
• numer dostępu NCBI: NP_001028935
• numer dostępu UniProt: Q3MI00
• Warunki transportu: dry ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... DNAJB1(3337)

Opis

Opis ogólny

DnaJ homolog subfamily B member 1 (UniProt Q3MI00) is encoded by the DNAJB1 gene (Gene ID 538426) in bovine species. Heat shock proteins (Hsps) are classified into six major families based on their relative molecular mass, Hsp110/hspH, Hsp90/HspC, Hsp70/HspA, Hsp60/HspD, Hsp40/DnaJ, and small heat shock proteins sHsp/HspB. Hsp40/DnaJB1 belongs to the Hsp40/DnaJ subfamily of heat shock proteins. Hsp40 acts as a co-chaperone with Hsp70 to facilitate protein folding, transport, and degradation. MK5 is reported to stimulate the ATP hydrolyse activity of Hsp40/Hsp70 complex by phosphorylating Hsp40/DnaJB1 C-terminal serine residues Ser149, Ser151, Ser171, resulting in an enhancement of Hsp40/DnaJB1-dependent suppression of heat shock factor 1-driven transcription. DnaJB1 is also shown to interact with MDM2 and prevent MDM2-mediated ubiquitination and proteasomal degradation of p53. DnaJB1 depletion promotes cancer cell growth as a result of p53 pathway inactivation.

Immunogen

Purified bovine DNAJB1.

Zastosowanie

Immunocytochemistry Analysis: 1-5 µg/mL from a representative lot detected heat shock-induced DNAJB1 upreguation in C6 rat glioma, B16 mouse melanoma, and K-562 human erythroleukemia cells (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences).
Flow Cytometry Analysis: 1-5 µg/mL from a representative lot detected heat shock-induced DNAJB1 upreguation in C6 rat glioma, B16 mouse melanoma, and K-562 human erythroleukemia cells (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences).
Western Blotting Analysis: 0.05-0.5 µg/mL from a representative lot detected heat shock-induced DNAJB1 upreguation in C6 rat glioma, B16 mouse melanoma, and K-562 human erythroleukemia cells (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences).

This Anti-DNAJB1/Hdj1 Antibody, clone J25 is validated for use in Western Blotting, Immunocytochemistry and Flow Cytometry for the detection of DNAJB1/Hdj1.

Jakość

Evaluated by Western Blotting in human K562 erythroblastoma cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected heat shock-induced DNAJB1/Hdj-1 upregulation in human K-562 erythroleukemia cells.

Opis wartości docelowych

~38 kDa observed

Postać fizyczna

Format: Purified

Inne uwagi

Concentration: Please refer to lot specific datasheet.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable