MABD85

Anti-LIMD1 Antibody, clone 3F2/C6

clone 3F2/C6, from mouse

• Synonim(y): LIM domain-containing protein 1
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• klon: 3F2/C6, monoclonal
• application: ICC; IHC; IP; WB
• reaktywność gatunkowa: human
• metody: immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• citations: 2

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 3F2/C6, monoclonal
• reaktywność gatunkowa: human
• metody: immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NP_055055
• numer dostępu UniProt: Q9UGP4
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... LIMD1(8994)

Opis

Opis ogólny

LIMD1 (LIM domains-containing protein 1) is a tumor suppressor and member of the Ajuba family of LIM domain-containing proteins. LIMD1 interacts with retinoblastoma protein (pRB), inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. Recently, LIMD1 has been implicated in regulating cellular processes involved in hypoxia. LIMD1 acts as a molecular scaffold, simultaneously binding the prolyl hydroxylases (PHDs) and von Hippel Lindau tumor suppressors (VHL), thereby assembling a PHD-LIMD1-VHL protein complex and creating an enzymatic niche that enables efficient degradation of HIF-1α.

Specyficzność

This antibody recognizes human LIMD1.

Immunogen

Recombinant protein corresponding to human LIMD1

Zastosowanie

Detect LIMD1 using this Anti-LIMD1 Antibody, clone 3F2/C6 validated for use in ICC, IHC, Immunoprecipitation, Western Blotting.

Research Category
Apoptosis & Cancer

Stem Cell Research

Research Sub Category
Oncoproteins

Oxidative Stress

This antibody has been shown to work in Immunocytochemical applications (Foxler, D., et al. (2012).
This antibody has been shown to work in Immunohistochemical applications (Sharp, T., et al. (2008).
This antibody has been shown to work in Immunoprecipitation applications (Courtesy of Dr. Tyson Sharp, Cancer Research, UK)

Jakość

Evaluated by Western Blot in H1299 cell lysates.
Western Blot Analysis: A 1:2000 dilution of this antibody detected LIMD1 in H1299 cell lysates.

Opis wartości docelowych

~72 kDa observed

Postać fizyczna

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
H1299 cell lysates.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable