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Key Documents

MABD24

Sigma-Aldrich

Anti-NANOG Antibody, clone 7F7.1

clone 7F7.1, from mouse

Synonim(y):

Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

100

forma przeciwciała

purified antibody

rodzaj przeciwciała

primary antibodies

klon

7F7.1, monoclonal

reaktywność gatunkowa

human

metody

immunocytochemistry: suitable
western blot: suitable

izotyp

IgG2aκ

numer dostępu NCBI

NP_079141

numer dostępu UniProt

Q9H9S0

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... NANOG(79923)

Opis ogólny

NANOG (Homeobox protein NANOG) is a member of the Nanog homeobox family of DNA-binding proteins. It is expressed in embryonic stem cells and confers pluripotency on these cells. Once embryonic stem cells become differentiated, NANOG expression is suppressed. NANOG is involved in the Hedgehog/Gli1 signaling pathway which has been implicated in the development and growth of various types of tumors.

Immunogen

GST-tagged recombinant protein corresponding to human NANOG.

Zastosowanie

Anti-NANOG Antibody, clone 7F7.1 is an antibody against NANOG for use in Western Blotting, ICC.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NANOG in H9 human embryonic stem cells.
Research Category
Stem Cell Research
Research Sub Category
Transcription Factors

Jakość

Evaluated by Western Blot in H9 human embryonic stem cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected NANOG on 10 µg of H9 human embryonic stem cell lysate.

Opis wartości docelowych

~39 kDa observed

Postać fizyczna

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
H9 human embryonic stem cell lysate

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Opis
Cennik

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Ambrin Fatima et al.
Stem cell research, 44, 101739-101739 (2020-03-04)
Incontinentia pigmenti (IP) is an X-linked dominant neuroectodermal dysplasia caused by loss-of-function mutations in the IKBKG gene. Using CRISPR/Cas9 technology, we generated an IKBKG knock-out iPSC line (KICRi002-A-1) on a 46,XY background. The iPSC line showed a normal karyotype, expressed
Jens Schuster et al.
Stem cell research, 57, 102577-102577 (2021-10-24)
Heterozygous variants in POLR2A, encoding the largest subunit of RNA polymerase II, cause severe neurodevelopmental and multisystem abnormalities in humans. Using CRISPR/Cas9 we generated the human iPSC line KICRi002A-5 with a heterozygous truncating 4 bp insertion in exon 5 of the
Jens Schuster et al.
Stem cell research, 39, 101523-101523 (2019-08-11)
Dravet syndrome (DS) is a childhood epilepsy syndrome caused by heterozygous mutations in the SCN1A gene encoding voltage-gated sodium channel Nav1.1. We generated iPSCs from fibroblasts of three DS patients carrying distinct SCN1A mutations (c.5502-5509dupGCTTGAAC, c.2965G>C and c.651C>G). The iPSC
Yifan Zhou et al.
Molecular autism, 7, 42-42 (2016-10-08)
Fragile X syndrome (FXS), a common cause of intellectual disability and autism, results from the expansion of a CGG-repeat tract in the 5' untranslated region of the FMR1 gene to >200 repeats. Such expanded alleles, known as full mutation (FM)
Jens Schuster et al.
Stem cell research, 38, 101474-101474 (2019-06-10)
Von Hippel-Lindau (VHL) syndrome is a familial cancer syndrome caused by mutations in the tumor suppressor gene VHL. We generated human iPSC lines from primary dermal fibroblasts of three VHL syndrome patients carrying distinct VHL germ line mutations (c.194C>G, c.194C>T
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