AB9220
Anti-Nanog Antibody
serum, Chemicon®
Synonim(y):
Anti-NANOG/STM1, Anti-STM1
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
serum
rodzaj przeciwciała
primary antibodies
klon
polyclonal
reaktywność gatunkowa
mouse, human
producent / nazwa handlowa
Chemicon®
metody
flow cytometry: suitable
western blot: suitable
moc wejściowa
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... NANOG(79923)
mouse ... Nanog(71950)
Opis ogólny
NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency. In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).
Specyficzność
Recognizes Nanog, human.
Immunogen
Synthetic peptide from human Nanog.
Zastosowanie
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
This Anti-Nanog Antibody is validated for use in FC, WB for the detection of Nanog.
Western blotting: 1:1,000-1:5,000. The antibody recognizes a protein of ~35 kDa on MBL-5 cell lysate. Additional bands of ~50 and ~60 kDa may be seen depending on sample and antibody dilution used.
Flow Cytometry: Cormier et al., 2006. Tissue Engineering 12:3233-3245.
Optimal working dilutions must be determined by the end user.
Flow Cytometry: Cormier et al., 2006. Tissue Engineering 12:3233-3245.
Optimal working dilutions must be determined by the end user.
Opis wartości docelowych
39 kDa (MW 34 kDa)
Postać fizyczna
Rabbit Serum. Liquid.
Unpurified
Przechowywanie i stabilność
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Komentarz do analizy
Control
Whole cell lysate from mouse embryonic stem cells
Whole cell lysate from mouse embryonic stem cells
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
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A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal.
Hu, G; Kim, J; Xu, Q; Leng, Y; Orkin, SH; Elledge, SJ
Genes & Development null
Guixiang Tan et al.
PloS one, 9(4), e92304-e92304 (2014-04-20)
Activation of signal transducer and activator of transcription 3 (Stat3) by leukemia inhibitory factor (LIF) is required for maintaining self-renewal and pluripotency of mouse embryonic stem cells (mESCs). Here, we have confirmed transcription factor Forkhead Box m1 (Foxm1) as a
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