MABC32

Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2

clone 11C9.2, from mouse

• Synonim(y): sequestosome 1, EBI3-associated protein of 60 kDa, Paget disease of bone 3, phosphotyrosine independent ligand for the Lck SH2 domain p62, oxidative stress induced like, EBI3-associated protein p60, Phosphotyrosine-independent ligand for the Lck SH2 doma
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: 11C9.2, monoclonal
• reaktywność gatunkowa: mouse, rat, human
• metody: flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• izotyp: IgMκ
• numer dostępu NCBI: NP_001135770
• numer dostępu UniProt: Q13501
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... SQSTM1(8878)

Opis

Opis ogólny

p62 is also known as sequestosome-1, and is a 62 kDa ras-GAP associated protein. p62 is thought to bind to src-family tyrosine kinases and may also provide a linker action for activated src-family kinases with their downstream effectors. p62 has an RNA-binding region and may have RNA-binding ability. p62 is associated with activation of transcription factor NF-κB, involved in cell differentiation, immune response and potassium channel regulation. p62 has recently been linked to extrinsic apoptosis and may be a key player in tumorigenesis.

Immunogen

GST-tagged recombinant protein corresponding to human p62 (Sequestosome-1).

Zastosowanie

Detect p62 (Sequestosome-1) using this Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2 validated for use in WB, FC & IC.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blot Analysis: 0.001 µg/mL from a previous lot detected p62 (Sequestosome-1) in 10 µg of PC12 cell lysate.

Flow Cytometry Analysis: 1.0 µg from a previous lot detected p62 (Sequestosome-1) in the staining of fixed and permeabilized HeLa cells.

Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected p62 (Sequestosome-1) in NIH/3T3, A431, and HeLa cells.

Jakość

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 0.001 µg/mL of this antibody detected p62 (Sequestosome-1) in 10 µg of A431 cell lysate.

Opis wartości docelowych

~47-60 kDa observed.
The calculated molecular weight of this protein is 47 kDa and also has an isoform at 38 kDa Due to modifications, this protein may be observed up to ~60 kDa in some lysates.

Postać fizyczna

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
A431 cell lysate

Inne uwagi

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable