MAB5404
Anti-Nitrotyrosine Antibody, clone 2A8.2
clone 2A8.2, Chemicon®, from mouse
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
2A8.2, monoclonal
reaktywność gatunkowa (przewidywana na podstawie homologii)
all
producent / nazwa handlowa
Chemicon®
metody
immunohistochemistry: suitable (paraffin)
western blot: suitable
izotyp
IgG2b
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... NOS1(4842)
Opis ogólny
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
Specyficzność
Reacts with nitrotyrosine.
Immunogen
Nitrated KLH.
Zastosowanie
Detect Nitrotyrosine using this Anti-Nitrotyrosine Antibody, clone 2A8.2 validated for use in IH(P) & WB.
Immunohistochemistry:
1:50-1:100 on paraffin embedded of a previous lot, 10% neutral buffer formalin fixed mouse liver (animal treated with Zymosan) and normal human cerebellum tissues. The tissues must be pretreated with heat induced epitope retrieval (HIER). Incubation with the MAB5404 was for 10 minutes and the reaction was developed with the Chemicon IHC-Select, HRP-DAB kit (Catalog number DAB150).
Optimal working dilutions must be determined by end user.
1:50-1:100 on paraffin embedded of a previous lot, 10% neutral buffer formalin fixed mouse liver (animal treated with Zymosan) and normal human cerebellum tissues. The tissues must be pretreated with heat induced epitope retrieval (HIER). Incubation with the MAB5404 was for 10 minutes and the reaction was developed with the Chemicon IHC-Select, HRP-DAB kit (Catalog number DAB150).
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Oxidative Stress
Oxidative Stress
Jakość
Evaluated by Western Blot on Nitrotyrosine Immunoblot Controls.
Western Blot Analysis:
1:500 dilution of this antibody detected Nitrotyrosine on 10 μg of Nitrotyrosine Immunoblot Controls (cat. # 12-354).
Western Blot Analysis:
1:500 dilution of this antibody detected Nitrotyrosine on 10 μg of Nitrotyrosine Immunoblot Controls (cat. # 12-354).
Postać fizyczna
Format: Purified
Protein A purified
Purified mouse monoclonal IgG2b liquid in buffer containing 0.1% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8ºC from date of receipt.
Komentarz do analizy
Control
Nitrated BSA
Nitrated BSA
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Epitope motif of an anti-nitrotyrosine antibody specific for tyrosine-nitrated peptides revealed by a combination of affinity approaches and mass spectrometry.
Mihaela Dr Gu?anu,Brindu?a-Alina Petre,Michael Przybylski
Journal of Peptide Science null
A Kiss et al.
British journal of pharmacology, 155(7), 1015-1024 (2008-10-11)
Exogenous peroxynitrite from nanomolar to micromolar concentrations exerts cardioprotection. Here, we have assessed its effects on ischaemia- and reperfusion-induced ventricular arrhythmias in vivo and a possible role for mitochondrial K(ATP) channels in these effects, using the channel inhibitor 5-hydroxydecanoate (5-HD).
Ben C Creelan et al.
OncoTargets and therapy, 10, 4239-4250 (2017-09-19)
Omaveloxolone is a semisynthetic oleanane triterpenoid that potently activates Nrf2 with subsequent antioxidant function. We conducted a first-in-human Phase I clinical trial (NCT02029729) with the primary objectives to determine the appropriate dose for Phase II studies, characterize pharmacokinetic and pharmacodynamic
When is mass spectrometry combined with affinity approaches essential? A case study of tyrosine nitration in proteins.
Petre, BA; Ulrich, M; Stumbaum, M; Bernevic, B; Moise, A; Doring, G; Przybylski, M
Journal of the American Society For Mass Spectrometry null
Deficiency of the Bax gene attenuates denervation-induced apoptosis.
P M Siu, S E Alway, P M Siu, S E Alway
Apoptosis null
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