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MAB4310

Sigma-Aldrich

Anti-CD133 Antibody, clone 13A4

clone 13A4, Chemicon®, from rat

Synonim(y):

Prominin-1, AC133 (human)

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

rat

Poziom jakości

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

13A4, monoclonal

reaktywność gatunkowa

mouse

spodziewany brak reakcji z

chicken, Drosophila, rat

producent / nazwa handlowa

Chemicon®

metody

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

moc wejściowa

sample type hematopoietic stem cell(s)
sample type neural stem cell(s)
sample type epithelial cells
sample type: mouse embryonic stem cell(s)
sample type mesenchymal stem cell(s)

izotyp

IgG1κ

przydatność

not suitable for immunohistochemistry (Paraffin)

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

dog ... Prom1(488816)
human ... PROM1(8842)
mouse ... Prom1(19126)
rat ... Prom1(60357)

Powiązane kategorie

Opis ogólny

Prominin is a novel plasma membrane protein with an N-terminal extracellular domain, five transmembrane segments flanking two short cytoplasmic loops and two large glycosylated extracellular domains, and a cytoplasmic C-terminal domain. DNA sequences from Caenorhabditis elegans predict the existence of a protein with the same features, suggesting that prominin is conserved between vertebrates and invertebrates. Prominin is found not only in the neuroepithelium but also in various other epithelia of the mouse embryo. In the adult mouse, prominin has been detected in the brain ependymal layer, and in kidney tubules. In these epithelia, prominin is specific to the apical surface, where it is selectively associated with microvilli and microvilli-related structures. Remarkably, upon expression in CHO cells, prominin is preferentially localized to plasma membrane protrusions such as filopodia, lamellipodia, and microspikes. These observations imply that prominin contains information to be targeted to, and/or retained in, plasma membrane protrusions rather than the planar cell surface. Moreover, results show that the mechanisms underlying targeting of membrane proteins to microvilli of epithelial cells and to plasma membrane protrusions of non-epithelial cells are highly related. {Weigmann, A et al.1997}. Also see {http://www.ncbi.nlm.nih.gov/prow/guide/438375806_g.htm} for more information on CD133 and prominin expression.

Specyficzność

Recognizes mouse Prominin-1 (sometimes also referred to as CD133 and, in the case of the human orthologue, as AC133), a 115-120 kDa pentaspan transmembrane (5-TM) domain glycoprotein. Prominin-1 is expressed on primitive cells such as hematopoietic stem and progenitor cells, neural & endothelial stem cells, retina and retinoblastoma, as well as developing epithelium. To date, the function and ligand of Prominin-1 are unknown.

This antibody can be used to isolate murine stem cells from brain and bone marrow.

The 13A4 antibody does not cross react with rat, human, chicken, or Drosophila antigen. The original paper is located at: http://www.pnas.org/cgi/reprint/94/23/12425.pdf

Immunogen

Telencephali of ten 12-day-old NMRI mouse embryos (E12, ~1 mg protein), which consist mostly of neuroepithelium, were homogenized in 1 ml PBS, mixed with crushed nitrocellulose filter (1 cm2 in 0.5 ml PBS) as adjuvant, and injected i.p. into a LouXSD rat. {Weigmann et al, PNAS 1997}

Zastosowanie

Anti-CD133 Antibody, clone 13A4 detects level of CD133 & has been published & validated for use in FC, IH, IP & WB.

Western Blot Analysis: A 1:1000 dilution from a representative lot detected CD133 in monkey kidney lysate.
Research Category
Stem Cell Research
Research Sub Category
Neural Stem Cells

Hematopoietic Stem Cells

Opis wartości docelowych

115 kDa

Postać fizyczna

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified

Przechowywanie i stabilność

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Komentarz do analizy

Control
Positive Control: Kidney tissue or membranes
Negative Control: Liver tissue or membranes

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Activation of the sonic hedgehog signaling pathway occurs in the CD133 positive cells of mouse liver cancer Hepa 1-6 cells.
Jeng, KS; Sheen, IS; Jeng, WJ; Yu, MC; Hsiau, HI; Chang, FY; Tsai, HH
OncoTargets and Therapy null
Ectopic expression of the WWOX gene suppresses stemness of human ovarian cancer stem cells.
Yan, HC; Xu, J; Fang, LS; Qiu, YY; Lin, XM; Huang, HX; Han, QY
Oncology Letters null
Zhibo Xia et al.
Molecular cancer research : MCR, 11(9), 1101-1111 (2013-06-14)
Glioma-initiating cells (GIC), which are characterized by their self-renewal capacity and tumorigenicity, were recently identified as a highly tumorigenic subpopulation of glioblastoma multiforme and are considered responsible for glioblastoma recurrence and chemo/radiation resistance. Previously, it was revealed that Wnt signaling
AC133 hematopoietic stem cell antigen: human homologue of mouse kidney prominin or distinct member of a novel protein family?
Corbeil, D, et al.
Blood, 91, 2625-2626 (1998)
Endothelinergic cells in the subependymal region of mice.
Mauricio M Casta?eda,Marisa A Cubilla,Martin M Lopez-Vicchi,Angela M Suburo
Brain Research null

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